Detailed information of TA system TA165238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 245072..245594
• Replicon: plasmid pRHB30-C02_2
• Accession: NZ_CP057331
• Organism: Klebsiella oxytoca strain RHB30-C02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: HVY70_RS29585
• Protein ID: WP_000220561.1
• Coordinates: 245313..245594 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: HVY70_RS29580
• Protein ID: WP_000121743.1
• Coordinates: 245072..245323 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY70_RS29560	240084..242474	-	2391	WP_000694550.1	Ig-like domain repeat protein	-
HVY70_RS29565	242547..242714	-	168	WP_000803860.1	hypothetical protein	-
HVY70_RS29570	243035..243217	+	183	WP_042634467.1	hypothetical protein	-
HVY70_RS29575	243251..243955	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
HVY70_RS29580	245072..245323	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
HVY70_RS29585	245313..245594	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY70_RS29590	245833..245925	-	93	Protein_258	IS6 family transposase	-
HVY70_RS29595	246091..247290	-	1200	WP_051124567.1	IS91 family transposase	-
HVY70_RS29600	247300..247488	-	189	WP_000957857.1	hypothetical protein	-
HVY70_RS29605	247608..249038	+	1431	Protein_261	IS4-like element IS50R family transposase	-
HVY70_RS29610	249067..249861	+	795	WP_000572405.1	aminoglycoside O-phosphotransferase APH(3')-IIa	-
HVY70_RS29615	249882..250118	+	237	Protein_263	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / blaTEM-1B / aph(3')-Ia / aac(3)-IVa / aph(4)-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / tet(A) / dfrA12 / qacE / sul1 / aph(3')-IIa	-	1..271498	271498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T165238 WP_000220561.1 NZ_CP057331:245313-245594 [Klebsiella oxytoca]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T165238 NZ_CP073771:1699459-1699980 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGGTACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTACAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCGTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGTTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT165238 WP_000121743.1 NZ_CP057331:245072-245323 [Klebsiella oxytoca]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT165238 NZ_CP073771:1699183-1699452 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGAATGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301