Detailed information of TA system TA165234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4549653..4550272
• Replicon: chromosome
• Accession: NZ_CP057330
• Organism: Klebsiella oxytoca strain RHB30-C02

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: HVY70_RS21430
• Protein ID: WP_004099646.1
• Coordinates: 4550054..4550272 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HVY70_RS21425
• Protein ID: WP_004099648.1
• Coordinates: 4549653..4550027 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY70_RS21415	4544809..4546002	+	1194	WP_004111040.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HVY70_RS21420	4546025..4549171	+	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit	-
HVY70_RS21425	4549653..4550027	+	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
HVY70_RS21430	4550054..4550272	+	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
HVY70_RS21435	4550433..4550999	+	567	WP_064344889.1	maltose O-acetyltransferase	-
HVY70_RS21440	4551136..4551606	+	471	WP_004099643.1	YlaC family protein	-
HVY70_RS21445	4551581..4553035	-	1455	WP_064344888.1	PLP-dependent aminotransferase family protein	-
HVY70_RS21450	4553138..4553836	+	699	WP_004099639.1	GNAT family N-acetyltransferase	-
HVY70_RS21455	4553833..4553973	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
HVY70_RS21460	4553973..4554236	-	264	WP_004099638.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T165234 WP_004099646.1 NZ_CP057330:4550054-4550272 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T165234 NZ_CP073771:c299814-299527 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT165234 WP_004099648.1 NZ_CP057330:4549653-4550027 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT165234 NZ_CP073771:c300123-299821 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3H713