Detailed information of TA system TA165132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 311466..312132
• Replicon: chromosome
• Accession: NZ_CP057258
• Organism: Citrobacter freundii strain RHB31-C16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HVY90_RS01485
• Protein ID: WP_181688683.1
• Coordinates: 311815..312132 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: HVY90_RS01480
• Protein ID: WP_003837894.1
• Coordinates: 311466..311762 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY90_RS01465	308642..309115	-	474	WP_003023524.1	transcription elongation factor GreB	-
HVY90_RS01470	309342..310061	+	720	WP_001157751.1	two-component system response regulator OmpR	-
HVY90_RS01475	310058..311410	+	1353	WP_096759268.1	two-component system sensor histidine kinase EnvZ	-
HVY90_RS01480	311466..311762	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
HVY90_RS01485	311815..312132	-	318	WP_181688683.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY90_RS01490	312256..313878	-	1623	WP_115257300.1	phosphoenolpyruvate carboxykinase (ATP)	-
HVY90_RS01495	314257..315975	+	1719	WP_181688684.1	DUF4153 domain-containing protein	-
HVY90_RS01500	316026..316904	-	879	WP_181688685.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12171.05 Da
• Isoelectric Point: 9.4575

>T165132 WP_181688683.1 NZ_CP057258:c312132-311815 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKTVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T165132 NZ_CP073718:717837-718214 [Escherichia coli]
ATGCAAACCCAACCACTATCCTCAACTCAGGAGGCTACACCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACACTCAACGACACTCCGTTCGGCAACGATGGCGTGATTCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGATCTGGTACGAACCGACCGTCGCGGTTTT
AACGCAGAAACACAGTCGCCTCTTCTCAGCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGACTGATGACCCGAAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGGGAGGTGCAGCCATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT165132 WP_003837894.1 NZ_CP057258:c311762-311466 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT165132 NZ_CP073718:717427-717786 [Escherichia coli]
TTGTCGAATGAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTACAGGCGGGCAACCGTCTGCATTATCTCGCTGACCGGGCCAACATCACCGGACAGTTCAGCGACG
CTGATTTACGGTATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAAT
CCCTGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5