Detailed information of TA system TA164970

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62311..62575
• Replicon: plasmid pRHB33-C04_3
• Accession: NZ_CP057217
• Organism: Escherichia fergusonii strain RHB33-C04

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: HVZ08_RS24115
• Protein ID: WP_024179514.1
• Coordinates: 62423..62575 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	62311..62373 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ08_RS24100	57550..59841	-	2292	WP_001289276.1	hypothetical protein	-
HVZ08_RS24105	59834..60904	-	1071	WP_001542508.1	IncI1-type conjugal transfer protein TrbB	-
HVZ08_RS24110	60923..62131	-	1209	WP_175237471.1	IncI1-type conjugal transfer protein TrbA	-
-	62311..62373	-	63	NuclAT_0	-	Antitoxin
-	62311..62373	-	63	NuclAT_0	-	Antitoxin
-	62311..62373	-	63	NuclAT_0	-	Antitoxin
-	62311..62373	-	63	NuclAT_0	-	Antitoxin
HVZ08_RS24115	62423..62575	+	153	WP_024179514.1	Hok/Gef family protein	Toxin
HVZ08_RS24120	62647..62898	-	252	WP_001291965.1	hypothetical protein	-
HVZ08_RS24125	63941..64150	-	210	WP_015059315.1	hemolysin expression modulator Hha	-
HVZ08_RS24130	64248..64862	-	615	WP_181667535.1	plasmid IncI1-type surface exclusion protein ExcA	-
HVZ08_RS24135	64938..67106	-	2169	WP_181667536.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..104967	104967

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5795.12 Da
• Isoelectric Point: 8.7948

>T164970 WP_024179514.1 NZ_CP057217:62423-62575 [Escherichia fergusonii]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T164970 NZ_CP073624:733993-734307 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT164970 NZ_CP057217:c62373-62311 [Escherichia fergusonii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin