Detailed information of TA system TA164854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4864220..4864974
• Replicon: chromosome
• Accession: NZ_CP057136
• Organism: Citrobacter freundii strain RHB36-C06

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HVZ46_RS22985
• Protein ID: WP_181618149.1
• Coordinates: 4864220..4864531 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVZ46_RS22990
• Protein ID: WP_181618150.1
• Coordinates: 4864528..4864974 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ46_RS22955	4859881..4860783	+	903	WP_115259809.1	formate dehydrogenase O subunit beta	-
HVZ46_RS22960	4860780..4861415	+	636	WP_096758858.1	formate dehydrogenase cytochrome b556 subunit	-
HVZ46_RS22965	4861412..4862341	+	930	WP_096758859.1	formate dehydrogenase accessory protein FdhE	-
HVZ46_RS22970	4862385..4862672	-	288	WP_079939299.1	helix-turn-helix domain-containing protein	-
HVZ46_RS22975	4862673..4862984	-	312	WP_181618147.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HVZ46_RS22980	4863205..4864134	+	930	WP_181618148.1	alpha/beta hydrolase	-
HVZ46_RS22985	4864220..4864531	+	312	WP_181618149.1	type II toxin-antitoxin system HigB family toxin	Toxin
HVZ46_RS22990	4864528..4864974	+	447	WP_181618150.1	helix-turn-helix domain-containing protein	Antitoxin
HVZ46_RS22995	4864989..4865930	-	942	WP_181618151.1	fatty acid biosynthesis protein FabY	-
HVZ46_RS23000	4865975..4866412	-	438	WP_181618152.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HVZ46_RS23005	4866409..4867281	-	873	WP_181618153.1	virulence factor BrkB family protein	-
HVZ46_RS23010	4867275..4867874	-	600	WP_181618154.1	glucose-1-phosphatase	-
HVZ46_RS23015	4867980..4868783	-	804	WP_181618155.1	DeoR/GlpR family DNA-binding transcription regulator	-
HVZ46_RS23020	4868817..4869713	-	897	WP_181618156.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12374.23 Da
• Isoelectric Point: 8.3851

>T164854 WP_181618149.1 NZ_CP057136:4864220-4864531 [Citrobacter freundii]
MHVISRKPFNNAMLIYPNYELALADLLNVLEKKTFTQPEEMKQFIPSLDNFKYRDEWWVIDVSGNSLRLISYIDFRSHKI
FVKHIVSHAEYDKLTAYYRSNKE

Nucleotide

• Length: 312 bp

>T164854 NZ_CP073360:268890-269120 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16633.84 Da
• Isoelectric Point: 6.0027

>AT164854 WP_181618150.1 NZ_CP057136:4864528-4864974 [Citrobacter freundii]
MRTHRQMDAASAKKIVDTFSDAVKNLPLLGEDRNDTEYRRALALVEFLVDHDDLENPLFELLSARISEYEKHAPEFNALN
QHLEKTPSGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKALTERFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT164854 NZ_CP073360:269117-269476 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA