Detailed information of TA system TA164803

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3447560..3448394
• Replicon: chromosome
• Accession: NZ_CP057104
• Organism: Escherichia fergusonii strain RHB38-C01

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2X7CAN1
• Locus tag: HVZ66_RS16790
• Protein ID: WP_039025883.1
• Coordinates: 3447560..3447937 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2X5LBZ2
• Locus tag: HVZ66_RS16795
• Protein ID: WP_039025882.1
• Coordinates: 3448026..3448394 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ66_RS16755	3443322..3443633	+	312	Protein_3268	hypothetical protein	-
HVZ66_RS16760	3443769..3443870	+	102	WP_000662204.1	hypothetical protein	-
HVZ66_RS16765	3444011..3444288	+	278	Protein_3270	tyrosine-type recombinase/integrase	-
HVZ66_RS16770	3444501..3445820	+	1320	WP_105284452.1	site-specific integrase	-
HVZ66_RS16775	3445913..3446761	-	849	WP_039025884.1	DUF4942 domain-containing protein	-
HVZ66_RS16780	3446858..3447055	-	198	WP_000772024.1	DUF957 domain-containing protein	-
HVZ66_RS16785	3447075..3447563	-	489	Protein_3274	hypothetical protein	-
HVZ66_RS16790	3447560..3447937	-	378	WP_039025883.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HVZ66_RS16795	3448026..3448394	-	369	WP_039025882.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVZ66_RS16800	3448444..3449088	-	645	WP_039025881.1	hypothetical protein	-
HVZ66_RS16805	3449103..3449324	-	222	WP_000692312.1	DUF987 domain-containing protein	-
HVZ66_RS16810	3449393..3449869	-	477	WP_001186745.1	RadC family protein	-
HVZ66_RS16815	3449884..3450369	-	486	WP_000214316.1	antirestriction protein	-
HVZ66_RS16820	3450460..3451278	-	819	WP_001234702.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14176.22 Da
• Isoelectric Point: 7.8523

>T164803 WP_039025883.1 NZ_CP057104:c3447937-3447560 [Escherichia fergusonii]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T164803 NZ_CP073302:c5415196-5414813 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13792.52 Da
• Isoelectric Point: 5.9582

>AT164803 WP_039025882.1 NZ_CP057104:c3448394-3448026 [Escherichia fergusonii]
VSDTFHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT164803 NZ_CP073302:c5415438-5415196 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A2X7CAN1

Antitoxin

• AlphaFold DB: A0A2X5LBZ2