Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2145592..2146154 Replicon chromosome
Accession NZ_CP057104
Organism Escherichia fergusonii strain RHB38-C01

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag HVZ66_RS10285 Protein ID WP_104919749.1
Coordinates 2145876..2146154 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HVZ66_RS10280 Protein ID WP_000781370.1
Coordinates 2145592..2145876 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ66_RS10265 2140951..2143998 + 3048 WP_015953395.1 formate dehydrogenase-N subunit alpha -
HVZ66_RS10270 2144011..2144895 + 885 WP_148048370.1 formate dehydrogenase N subunit beta -
HVZ66_RS10275 2144888..2145541 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
HVZ66_RS10280 2145592..2145876 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HVZ66_RS10285 2145876..2146154 - 279 WP_104919749.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HVZ66_RS10290 2146340..2147350 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
HVZ66_RS10295 2147484..2149181 - 1698 WP_000433464.1 malate dehydrogenase -
HVZ66_RS10300 2149338..2149475 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HVZ66_RS10305 2149577..2149792 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HVZ66_RS10310 2150134..2150565 + 432 WP_181591473.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T164801 WP_104919749.1 NZ_CP057104:c2146154-2145876 [Escherichia fergusonii]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADLDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T164801 NZ_CP073302:c4934862-4934578 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT164801 WP_000781370.1 NZ_CP057104:c2145876-2145592 [Escherichia fergusonii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT164801 NZ_CP073302:c4935094-4934852 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References