Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5105546..5106171 Replicon chromosome
Accession NZ_CP057034
Organism Klebsiella pneumoniae strain RHB39-C24

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HVZ90_RS24890 Protein ID WP_002882817.1
Coordinates 5105546..5105929 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HVZ90_RS24895 Protein ID WP_004150355.1
Coordinates 5105929..5106171 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ90_RS24875 5102912..5103814 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HVZ90_RS24880 5103811..5104446 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HVZ90_RS24885 5104443..5105372 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HVZ90_RS24890 5105546..5105929 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HVZ90_RS24895 5105929..5106171 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HVZ90_RS24900 5106376..5107293 + 918 WP_065803683.1 alpha/beta hydrolase -
HVZ90_RS24905 5107307..5108248 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HVZ90_RS24910 5108293..5108730 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HVZ90_RS24915 5108727..5109587 - 861 WP_004146232.1 virulence factor BrkB family protein -
HVZ90_RS24920 5109581..5110180 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T164667 WP_002882817.1 NZ_CP057034:c5105929-5105546 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T164667 NZ_CP073021:9948-10373 [Synechocystis sp. PCC 6803]
GTGAAAGTACTTCTCGACACCAATATTGTTCTCGACTTACTTTTAGAGAGAGAACCGTTTATCGAAGAAACCATAGCCAT
CTTTGAAGAAATCGAGCAGGGAAACATCGAAGCTTACATTGCGGCTACAACAGTTACCAATATTTTTTACATCATTCGTA
AGAACAAAAGCCGCGAGGTGGCGCTTATAGCTGTTAAGCGACTACTGATTGGACTACAACTATGCCCCGTGAATCTACAA
GTTATCGAAGTTGCCCTCGACATCAATCTAAGAGACTTTGAAGATAGTGTCCAATTGGCATGTGCCACAGTTAACCAACT
TACTGCAATCATTACCCGTGACACCAAAGACTTTGTTGAGAGTCCTTTGCCTATTTACTCCCCTGCGGAATGTTTATATC
GGGTTAAGACCAATAAGCTTGATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT164667 WP_004150355.1 NZ_CP057034:c5106171-5105929 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT164667 NZ_CP073021:9713-9940 [Synechocystis sp. PCC 6803]
ATGACAGCGCAAGAAAAACTCTACCAACTAATTCAAACTCTGCCAGAGAAGGAAGTTAGCGAAATACTCAATTTTGCCGA
GTCGCTTTGGCCAAAAGTCTCTAGCCCCAAAATTCCCGCCGGCACCCTTACCGGACTGCGGGGCATTGCCAAACCAACGG
GCCCCGTACCTAGCGACGCTGAGTTACAAAGGCAATACACTGACTACCTAAAAGAGAAGTATCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References