Detailed information of TA system TA164620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 611809..612608
• Replicon: chromosome
• Accession: NZ_CP056968
• Organism: Escherichia fergusonii strain RHB41-C15

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: HV010_RS02960
• Protein ID: WP_000347270.1
• Coordinates: 611809..612273 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: HV010_RS02965
• Protein ID: WP_181588313.1
• Coordinates: 612273..612608 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV010_RS02930	606810..607244	-	435	WP_181588311.1	PTS sugar transporter subunit IIA	-
HV010_RS02935	607262..608140	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HV010_RS02940	608130..608909	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HV010_RS02945	608920..609393	-	474	WP_032303829.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HV010_RS02950	609416..610696	-	1281	WP_181588312.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HV010_RS02955	610945..611754	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HV010_RS02960	611809..612273	-	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HV010_RS02965	612273..612608	-	336	WP_181588313.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HV010_RS02970	612757..614328	-	1572	WP_181588314.1	galactarate dehydratase	-
HV010_RS02975	614799..615569	+	771	WP_001058567.1	2-dehydro-3-deoxyglucarate aldolase	-
HV010_RS02980	615594..616484	+	891	WP_000178100.1	2-hydroxy-3-oxopropionate reductase	-
HV010_RS02990	617139..617507	-	369	WP_181588315.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T164620 WP_000347270.1 NZ_CP056968:c612273-611809 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T164620 NZ_CP073002:741480-741965 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12282.88 Da
• Isoelectric Point: 4.3398

>AT164620 WP_181588313.1 NZ_CP056968:c612608-612273 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEEEDPTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT164620 NZ_CP073002:741190-741483 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90