Detailed information of TA system TA164495

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4716976..4717764
• Replicon: chromosome
• Accession: NZ_CP056910
• Organism: Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HV030_RS23110
• Protein ID: WP_181638367.1
• Coordinates: 4717387..4717764 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV030_RS23105
• Protein ID: WP_181638366.1
• Coordinates: 4716976..4717335 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV030_RS23050	4711995..4712498	+	504	Protein_4521	HTH domain-containing protein	-
HV030_RS23055	4712565..4712876	+	312	WP_023336630.1	hypothetical protein	-
HV030_RS23060	4712923..4713393	+	471	WP_181638360.1	hypothetical protein	-
HV030_RS23065	4713439..4713951	+	513	WP_181638361.1	DUF4234 domain-containing protein	-
HV030_RS23070	4714031..4714252	+	222	WP_101700256.1	hypothetical protein	-
HV030_RS23075	4714309..4714542	+	234	WP_101700257.1	DUF905 domain-containing protein	-
HV030_RS23080	4714654..4715472	+	819	WP_181638362.1	DUF945 domain-containing protein	-
HV030_RS23085	4715472..4715726	+	255	WP_101700259.1	hypothetical protein	-
HV030_RS23090	4715743..4716228	+	486	WP_181638363.1	antirestriction protein	-
HV030_RS23095	4716239..4716718	+	480	WP_181638364.1	DNA repair protein RadC	-
HV030_RS23100	4716732..4716953	+	222	WP_181638365.1	DUF987 domain-containing protein	-
HV030_RS23105	4716976..4717335	+	360	WP_181638366.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV030_RS23110	4717387..4717764	+	378	WP_181638367.1	TA system toxin CbtA family protein	Toxin
HV030_RS23115	4717761..4718252	+	492	WP_181638368.1	hypothetical protein	-
HV030_RS23120	4718285..4718488	+	204	WP_181638369.1	hypothetical protein	-
HV030_RS23125	4718558..4719403	+	846	WP_181638370.1	DUF4942 domain-containing protein	-
HV030_RS23130	4719415..4719651	+	237	WP_181638371.1	hypothetical protein	-
HV030_RS23135	4720080..4721429	-	1350	WP_181638749.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13922.78 Da
• Isoelectric Point: 9.0116

>T164495 WP_181638367.1 NZ_CP056910:4717387-4717764 [Citrobacter freundii]
MQTRQLSSTREASPRPTPVEIWQTLLTYLLERHYGLSLNDTQFGDGNVIHQHIDAGISLADALNFLVEKSELVRIDRPGF
SIQHQSPFISAIDILRARKATGLMQRTGYKAVTCAISGQSSRGQQ

Nucleotide

• Length: 378 bp

>T164495 NZ_CP072961:c298369-298082 [Enterobacter sp. MGH 14]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13180.08 Da
• Isoelectric Point: 6.7424

>AT164495 WP_181638366.1 NZ_CP056910:4716976-4717335 [Citrobacter freundii]
MQNPTNVASHDITEPWWGLKPAVIPCFGARLVQEGNRLHYLADRASITGTFSNADLRHLDQAFPVLMKQLELMLLSGELN
PRHQHCVTLYAKGLTCESDTLGSHGYVYLAIYPTSAITA

Nucleotide

• Length: 360 bp

>AT164495 NZ_CP072961:c298678-298376 [Enterobacter sp. MGH 14]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTGGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA