Detailed information of TA system TA164492

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4121597..4122276
• Replicon: chromosome
• Accession: NZ_CP056910
• Organism: Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A8B3US07
• Locus tag: HV030_RS20265
• Protein ID: WP_007777710.1
• Coordinates: 4121935..4122276 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV030_RS20260
• Protein ID: WP_181638320.1
• Coordinates: 4121597..4121914 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV030_RS20215	4117036..4117857	+	822	WP_001548934.1	DUF945 domain-containing protein	-
HV030_RS20220	4118075..4118776	+	702	WP_001548933.1	WYL domain-containing protein	-
HV030_RS20225	4118813..4119049	+	237	WP_001115854.1	hypothetical protein	-
HV030_RS20230	4119049..4119492	+	444	WP_000824224.1	hypothetical protein	-
HV030_RS20235	4119516..4119983	+	468	WP_115799765.1	hypothetical protein	-
HV030_RS20240	4120060..4120299	+	240	WP_000194654.1	DUF905 domain-containing protein	-
HV030_RS20245	4120397..4120855	+	459	WP_001547766.1	antirestriction protein	-
HV030_RS20250	4120871..4121347	+	477	WP_000811693.1	RadC family protein	-
HV030_RS20255	4121356..4121577	+	222	WP_001548171.1	DUF987 domain-containing protein	-
HV030_RS20260	4121597..4121914	+	318	WP_181638320.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV030_RS20265	4121935..4122276	+	342	WP_007777710.1	TA system toxin CbtA family protein	Toxin
HV030_RS20275	4122853..4124106	-	1254	WP_181540019.1	glutamate-5-semialdehyde dehydrogenase	-
HV030_RS20280	4124118..4125221	-	1104	WP_096758188.1	glutamate 5-kinase	-
HV030_RS20285	4125510..4126565	+	1056	WP_096758189.1	phosphoporin PhoE	-
HV030_RS20290	4126622..4127023	-	402	WP_096758190.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12934.88 Da
• Isoelectric Point: 8.0308

>T164492 WP_007777710.1 NZ_CP056910:4121935-4122276 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLQAVDILRARQATGLLQQSRSNVVR

Nucleotide

• Length: 342 bp

>T164492 NZ_CP072954:36269-36556 [Enterobacter sp. MGH 3]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12018.67 Da
• Isoelectric Point: 7.3723

>AT164492 WP_181638320.1 NZ_CP056910:4121597-4121914 [Citrobacter freundii]
MTHINWGLQRNITPRLGARLVQEGNRLHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVIMYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT164492 NZ_CP072954:36030-36269 [Enterobacter sp. MGH 3]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAAGCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A8B3US07