Detailed information of TA system TA164491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3821888..3822508
• Replicon: chromosome
• Accession: NZ_CP056910
• Organism: Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HV030_RS18745
• Protein ID: WP_002892050.1
• Coordinates: 3822290..3822508 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV030_RS18740
• Protein ID: WP_115259201.1
• Coordinates: 3821888..3822262 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV030_RS18730	3817034..3818227	+	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV030_RS18735	3818250..3821399	+	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
HV030_RS18740	3821888..3822262	+	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
HV030_RS18745	3822290..3822508	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HV030_RS18750	3822890..3823363	+	474	WP_181539966.1	YlaC family protein	-
HV030_RS18755	3823437..3823577	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HV030_RS18760	3823579..3823839	-	261	WP_115259215.1	type B 50S ribosomal protein L31	-
HV030_RS18765	3824024..3825577	+	1554	WP_181638246.1	EAL domain-containing protein	-
HV030_RS18770	3825631..3825984	-	354	WP_115259217.1	DUF1428 family protein	-
HV030_RS18775	3826067..3826678	-	612	WP_174360590.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T164491 WP_002892050.1 NZ_CP056910:3822290-3822508 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T164491 NZ_CP072953:c3890449-3890039 [Enterobacter sp. MGH 3]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTGGTGTTTGATAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGGGATCTGCGCCGGCTAATCGTGCAACAGCCAA
CGCAGGAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT164491 WP_115259201.1 NZ_CP056910:3821888-3822262 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT164491 NZ_CP072953:c3890696-3890430 [Enterobacter sp. MGH 3]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2