Detailed information of TA system TA164479

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1216622..1217289
• Replicon: chromosome
• Accession: NZ_CP056910
• Organism: Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HV030_RS05920
• Protein ID: WP_181630016.1
• Coordinates: 1216622..1216951 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0F6RHY7
• Locus tag: HV030_RS05925
• Protein ID: WP_046495841.1
• Coordinates: 1216972..1217289 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV030_RS05905	1212451..1214355	+	1905	WP_181540491.1	5-dehydro-2-deoxygluconokinase	-
HV030_RS05910	1214370..1215260	+	891	WP_096756056.1	myo-inosose-2 dehydratase	-
HV030_RS05915	1215270..1216088	+	819	WP_181506668.1	5-deoxy-glucuronate isomerase	-
HV030_RS05920	1216622..1216951	-	330	WP_181630016.1	TA system toxin CbtA family protein	Toxin
HV030_RS05925	1216972..1217289	-	318	WP_046495841.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV030_RS05930	1217308..1217529	-	222	WP_000691995.1	DUF987 domain-containing protein	-
HV030_RS05935	1217538..1218014	-	477	WP_046495838.1	RadC family protein	-
HV030_RS05940	1218030..1218488	-	459	WP_128347215.1	antirestriction protein	-
HV030_RS05945	1218572..1218811	-	240	WP_054626379.1	DUF905 domain-containing protein	-
HV030_RS05950	1218911..1219819	-	909	WP_064761968.1	hypothetical protein	-
HV030_RS05955	1219888..1220055	-	168	Protein_1165	DUF4339 domain-containing protein	-
HV030_RS05960	1220093..1220788	-	696	WP_054626378.1	hypothetical protein	-
HV030_RS05965	1221015..1221842	-	828	WP_054626377.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12392.27 Da
• Isoelectric Point: 8.9791

>T164479 WP_181630016.1 NZ_CP056910:c1216951-1216622 [Citrobacter freundii]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRNGF
SWQEQTPYISVVDILRARRSTGLLKNNVK

Nucleotide

• Length: 330 bp

>T164479 NZ_CP072949:c4811894-4811610 [Klebsiella pneumoniae MGH 39]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11869.49 Da
• Isoelectric Point: 6.6245

>AT164479 WP_046495841.1 NZ_CP056910:c1217289-1216972 [Citrobacter freundii]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Nucleotide

• Length: 318 bp

>AT164479 NZ_CP072949:c4812126-4811884 [Klebsiella pneumoniae MGH 39]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0F6RHY7