Detailed information of TA system TA164476

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 793051..793795
• Replicon: chromosome
• Accession: NZ_CP056910
• Organism: Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1Q8YX07
• Locus tag: HV030_RS03855
• Protein ID: WP_044349292.1
• Coordinates: 793051..793428 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV030_RS03860
• Protein ID: WP_170960574.1
• Coordinates: 793469..793795 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV030_RS03825	788823..789320	+	498	WP_181625697.1	type VI secretion system contractile sheath small subunit	-
HV030_RS03830	789386..790075	+	690	Protein_751	type VI secretion system contractile sheath large subunit	-
HV030_RS03835	789986..790945	-	960	WP_181625698.1	PAAR domain-containing protein	-
HV030_RS03840	791341..791577	-	237	WP_181638371.1	hypothetical protein	-
HV030_RS03845	791589..792434	-	846	WP_181638370.1	DUF4942 domain-containing protein	-
HV030_RS03850	792563..793054	-	492	WP_044349294.1	hypothetical protein	-
HV030_RS03855	793051..793428	-	378	WP_044349292.1	TA system toxin CbtA family protein	Toxin
HV030_RS03860	793469..793795	-	327	WP_170960574.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV030_RS03865	793853..794074	-	222	WP_044349288.1	DUF987 domain-containing protein	-
HV030_RS03870	794088..794567	-	480	WP_044349286.1	DNA repair protein RadC	-
HV030_RS03875	794579..795022	-	444	WP_181638471.1	antirestriction protein	-
HV030_RS03880	795102..795359	-	258	WP_044349282.1	DUF905 domain-containing protein	-
HV030_RS03885	795356..796180	-	825	WP_065805884.1	DUF945 domain-containing protein	-
HV030_RS03890	796718..797350	+	633	WP_181638472.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13849.76 Da
• Isoelectric Point: 6.9771

>T164476 WP_044349292.1 NZ_CP056910:c793428-793051 [Citrobacter freundii]
MKILSDSPTRAAPSRPSPVEIWQTLLTYLLDQHYGLTLNDTPFGHDGVIQEHIGTGITLCDAVNLIVEKYKLVRIDRHGF
SADTQSPLIGSIDILRARKETGLMTRHGYRAITDITTGKYSEAGQ

Nucleotide

• Length: 378 bp

>T164476 NZ_CP072949:4090710-4090928 [Klebsiella pneumoniae MGH 39]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12101.76 Da
• Isoelectric Point: 6.4639

>AT164476 WP_170960574.1 NZ_CP056910:c793795-793469 [Citrobacter freundii]
ISEPWWGLRRSVSPCFGARLVQEGNRLNYLADRANFDGQFCDADLRHLDQAFPVLMKQLELMLTSGELNPRHQHCVTLYA
KGLTCEADTLGSHGYLYLAIYTTPATAA

Nucleotide

• Length: 327 bp

>AT164476 NZ_CP072949:4090309-4090683 [Klebsiella pneumoniae MGH 39]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAGGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A1Q8YX07