Detailed information of TA system TA164388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 154717..155462
• Replicon: plasmid pRHBSTW-00066_2
• Accession: NZ_CP056879
• Organism: Klebsiella pneumoniae strain RHBSTW-00066

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: HV051_RS26480
• Protein ID: WP_032408901.1
• Coordinates: 154971..155462 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: HV051_RS26475
• Protein ID: WP_014386183.1
• Coordinates: 154717..154983 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV051_RS26425	150270..150683	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
HV051_RS26430	150684..150962	-	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
HV051_RS26435	150952..151271	-	320	Protein_157	type II toxin-antitoxin system RelE/ParE family toxin	-
HV051_RS26440	151352..151576	-	225	WP_014386189.1	hypothetical protein	-
HV051_RS26445	151587..151799	-	213	WP_014386188.1	hypothetical protein	-
HV051_RS26450	151861..152187	-	327	WP_014386187.1	hypothetical protein	-
HV051_RS26455	152824..153174	-	351	WP_014386186.1	hypothetical protein	-
HV051_RS26460	153171..153443	-	273	WP_032408902.1	hypothetical protein	-
HV051_RS26465	153633..154118	+	486	WP_014386185.1	hypothetical protein	-
HV051_RS26470	154362..154520	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
HV051_RS26475	154717..154983	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
HV051_RS26480	154971..155462	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
HV051_RS26485	155903..156154	-	252	WP_032408900.1	aldo/keto reductase	-
HV051_RS26490	156351..157943	-	1593	Protein_168	IS66 family transposase	-
HV051_RS26495	157974..158324	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
HV051_RS26500	158321..158761	-	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
HV051_RS26505	159023..159778	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(D) / blaSHV-12	-	1..199477	199477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T164388 WP_032408901.1 NZ_CP056879:154971-155462 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T164388 NZ_CP072904:51168-51440 [Bartonella henselae]
ATGAAGCTCGTTTGGACACGGATAGCGCATTACGATCGTCAAAAGATACGGGAATATATCGCGCAAGAAAATCCTTCTGC
TGCATTAGTATTCGACAAGCTTTTATCTAAAAAAGTAGAAAGACTCATTAGCTTTCCTAATCTTGGTCGTGTTGGCCGAA
TTAGGAATACGCGCGAGCTTATTGTGCGTAAGAATTATATTATGATTTATGATGTTTCTAATGGCACTATACGGGTTTTG
CGCATACTTCACGCAAAACAAAAGTGGCCATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT164388 WP_014386183.1 NZ_CP056879:154717-154983 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT164388 NZ_CP072904:50881-51171 [Bartonella henselae]
ATGGCTGAAACAACATTTACCTTTCGCGTTGATGATGTATTAAAAAGTGAATTTTCTCGGGCTGCAAAAGCATGTGATCG
ATCTGCTGCACAGCTCTTACGGGATTACATGCGTGATATTGTAAAAGAACAAAAAGAAAAGATAGCGCATGAATTATGGT
TTCAAGAACAAGTCCAACTTGGATTGAATTCTGCCAACGCAGGTGATGTAATACCTTTTGAAGAAATTGAAACAGAAGCA
CAAGCATGGCGCTTTGAAATACAACGCAAGCTGAAGACATCAGATTCATGA

Structures

Toxin

• AlphaFold DB: A0A331KSM6