Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4478880..4479690 Replicon chromosome
Accession NZ_CP056854
Organism Klebsiella pneumoniae strain RHBSTW-00113

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HV075_RS21930 Protein ID WP_004178461.1
Coordinates 4478880..4479413 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HV075_RS21935 Protein ID WP_002887278.1
Coordinates 4479424..4479690 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV075_RS21925 4477711..4478832 + 1122 WP_023280822.1 cupin domain-containing protein -
HV075_RS21930 4478880..4479413 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
HV075_RS21935 4479424..4479690 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HV075_RS21940 4479793..4481226 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
HV075_RS21945 4481216..4481899 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HV075_RS21950 4482072..4483457 + 1386 WP_032412712.1 efflux transporter outer membrane subunit -
HV075_RS21955 4483475..4483819 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T164362 WP_004178461.1 NZ_CP056854:c4479413-4478880 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T164362 NZ_CP072901:c262101-261832 [Bartonella henselae]
TTGGCTTGGACGATTAGATATGAAAAAAAAGCTCTTAGTTTTTTAAAAAAATGCGATAAAAAAGAAGCACGGCGGATTGT
TGATTTTTTAGATCAACACGTTGCTCCTCTTGAAGATGTGCGTGTAATAGGTAAGCCCTTAAAAGGACAATTCTCAGGTT
TATGGAGATATCGTGTAGGAGATTACAGGATACTTTGTGAACTCTATGATAAGGAGCTTGTCGTATTAGTTTTGGCTGTT
GGACATAGAAAAAATATATATAAAGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT164362 WP_002887278.1 NZ_CP056854:c4479690-4479424 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT164362 NZ_CP072901:c262312-262085 [Bartonella henselae]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAGGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References