Detailed information of TA system TA164229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 30271..30914
• Replicon: plasmid pctg1
• Accession: NZ_CP056780
• Organism: Enterobacter cloacae strain D41-sc-1712200

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HWQ17_RS22975
• Protein ID: WP_148422427.1
• Coordinates: 30271..30687 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A5P6KGW8
• Locus tag: HWQ17_RS22980
• Protein ID: WP_021312767.1
• Coordinates: 30684..30914 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ17_RS22950	26290..27477	-	1188	WP_148422431.1	hypothetical protein	-
HWQ17_RS22955	27482..27730	-	249	WP_148422430.1	hypothetical protein	-
HWQ17_RS22960	28065..28829	-	765	WP_148422429.1	NAD(P)-binding domain-containing protein	-
HWQ17_RS22965	28847..29581	-	735	WP_148422428.1	NAD(P)-binding domain-containing protein	-
HWQ17_RS22970	29612..30046	-	435	WP_174592426.1	MarR family transcriptional regulator	-
HWQ17_RS22975	30271..30687	-	417	WP_148422427.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWQ17_RS22980	30684..30914	-	231	WP_021312767.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HWQ17_RS22985	31507..31821	+	315	WP_148422426.1	hypothetical protein	-
HWQ17_RS22990	31880..32644	+	765	WP_148422425.1	hypothetical protein	-
HWQ17_RS22995	32697..33044	+	348	WP_148422424.1	hypothetical protein	-
HWQ17_RS23000	33041..33820	+	780	WP_148422423.1	site-specific integrase	-
HWQ17_RS23005	34481..35113	+	633	WP_148422422.1	ParA family protein	-
HWQ17_RS23010	35113..35475	+	363	WP_148422421.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..87751	87751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15107.47 Da
• Isoelectric Point: 7.2449

>T164229 WP_148422427.1 NZ_CP056780:c30687-30271 [Enterobacter cloacae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCTRLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLTLEDWVN

Nucleotide

• Length: 417 bp

>T164229 NZ_CP072848:c399997-399707 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8722.81 Da
• Isoelectric Point: 4.4692

>AT164229 WP_021312767.1 NZ_CP056780:c30914-30684 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT164229 NZ_CP072848:c400235-399987 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A5P6KGW8