Detailed information of TA system TA164214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1565125..1565745
• Replicon: chromosome
• Accession: NZ_CP056779
• Organism: Enterobacter cloacae strain D41-sc-1712200

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A837FFM2
• Locus tag: HWQ17_RS07440
• Protein ID: WP_015571250.1
• Coordinates: 1565527..1565745 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HWQ17_RS07435
• Protein ID: WP_148420309.1
• Coordinates: 1565125..1565499 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ17_RS07425	1560253..1561446	+	1194	WP_148420311.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HWQ17_RS07430	1561469..1564615	+	3147	WP_148420310.1	multidrug efflux RND transporter permease subunit AcrB	-
HWQ17_RS07435	1565125..1565499	+	375	WP_148420309.1	Hha toxicity modulator TomB	Antitoxin
HWQ17_RS07440	1565527..1565745	+	219	WP_015571250.1	hemolysin expression modulator Hha	Toxin
HWQ17_RS07445	1565953..1566504	+	552	WP_148420308.1	maltose O-acetyltransferase	-
HWQ17_RS07450	1566621..1567088	+	468	WP_148420307.1	hypothetical protein	-
HWQ17_RS07455	1567124..1567264	-	141	WP_034458060.1	type B 50S ribosomal protein L36	-
HWQ17_RS07460	1567267..1567527	-	261	WP_148420306.1	type B 50S ribosomal protein L31	-
HWQ17_RS07465	1567755..1569323	+	1569	WP_148420305.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T164214 WP_015571250.1 NZ_CP056779:1565527-1565745 [Enterobacter cloacae]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T164214 NZ_CP072843:c1223568-1222822 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGAGTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14513.34 Da
• Isoelectric Point: 4.8886

>AT164214 WP_148420309.1 NZ_CP056779:1565125-1565499 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPMSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT164214 NZ_CP072843:c1222821-1222651 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A837FFM2