Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 49060..49703 Replicon plasmid pRHBSTW-00216_2
Accession NZ_CP056752
Organism Enterobacter hormaechei strain RHBSTW-00216

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag HV128_RS23295 Protein ID WP_000754567.1
Coordinates 49287..49703 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag HV128_RS23290 Protein ID WP_023293777.1
Coordinates 49060..49290 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV128_RS23265 44897..45010 + 114 WP_032427600.1 hypothetical protein -
HV128_RS23270 45139..45396 + 258 WP_000764642.1 hypothetical protein -
HV128_RS23275 45454..46242 - 789 WP_022631000.1 site-specific integrase -
HV128_RS23280 46262..46933 - 672 WP_024140048.1 hypothetical protein -
HV128_RS23290 49060..49290 + 231 WP_023293777.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV128_RS23295 49287..49703 + 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
HV128_RS23300 50054..51022 - 969 WP_016151349.1 IS5 family transposase -
HV128_RS23305 51182..51445 + 264 WP_001295708.1 hypothetical protein -
HV128_RS23310 51639..51920 + 282 WP_023332908.1 helix-turn-helix domain-containing protein -
HV128_RS23315 51955..52524 + 570 WP_023332907.1 small heat shock protein sHSP20 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..58103 58103
- inside IScluster/Tn - - 47649..51022 3373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T164142 WP_000754567.1 NZ_CP056752:49287-49703 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T164142 NZ_CP072801:c1560162-1559809 [Thiothrix litoralis]
ATGGTAAAGCGTGGCGGATTTGAACGCGGCGACATTGTGAGAGTCAGCTTTAACCCGACAGTGGGGCGTGAGATGCAAGG
CGACATGCGCCCTGCACTGGTACTTTCCACCCGTGAATTCAATAATACTGTCGGCACTGCATTGGTAGCACCGATTACCC
AAGGCGGCAACCTTGCGCGGGTGGCGGGTTTCGCCGTGCCATTGATGGGCAGCGGCACAGAAACGCAGGGCGTTGTTCTG
GTTAGTGCAGTGCGGACACTTGACCTAGTGGGCAGGGGGGCAAGGCGCATAGAAACCGCCCCTGATAGCATTGTCGATGA
AGTCCTAGAAATACTCGCGGGCATCATTGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT164142 WP_023293777.1 NZ_CP056752:49060-49290 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT164142 NZ_CP072801:c1560422-1560156 [Thiothrix litoralis]
ATGACCACCGCAACACTACGCACCGTCGGTAATTCCGTCGCTGTGGTAATCCCTAGACAATGGTTGTCTATGCTGGGGCT
GGAAGCAGGCGCGAAAGTAGAGCTAAGTGTCGAAGGCGGCAAACTCACCCTACAGCCAAAAGCAGCGGCTAGGCGCAAAA
AATACAAACTAGCCGACCTGTTAGCTAAGTGTGACCCATCCGCACCGATACCAACAGCAGTAACCGAATGGGACAACATG
CCAGCGGCAGGGAATGAAGTATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References