Detailed information of TA system TA163975

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3905601..3906280
• Replicon: chromosome
• Accession: NZ_CP056707
• Organism: Enterobacter hormaechei strain RHBSTW-00564

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A411GL63
• Locus tag: HV236_RS18835
• Protein ID: WP_016243829.1
• Coordinates: 3905601..3905942 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV236_RS18840
• Protein ID: WP_150320518.1
• Coordinates: 3905963..3906280 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV236_RS18790	3900785..3900934	-	150	WP_017382390.1	DUF3927 family protein	-
HV236_RS18795	3900934..3901359	-	426	WP_181522229.1	tellurite resistance TerB family protein	-
HV236_RS18800	3901429..3901629	-	201	WP_181522230.1	hypothetical protein	-
HV236_RS18805	3901619..3901843	-	225	WP_181522231.1	DUF2724 domain-containing protein	-
HV236_RS18810	3901851..3902360	-	510	WP_181522232.1	phage regulatory CII family protein	-
HV236_RS18815	3902395..3902631	-	237	WP_062856346.1	hypothetical protein	-
HV236_RS18820	3902733..3903347	+	615	WP_181522233.1	phage repressor protein CI	-
HV236_RS18825	3903359..3904258	+	900	WP_181522234.1	NAD-dependent DNA ligase	-
HV236_RS18830	3904268..3905353	+	1086	WP_181522235.1	tyrosine-type recombinase/integrase	-
HV236_RS18835	3905601..3905942	-	342	WP_016243829.1	TA system toxin CbtA family protein	Toxin
HV236_RS18840	3905963..3906280	-	318	WP_150320518.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV236_RS18845	3906299..3906520	-	222	WP_111975972.1	DUF987 domain-containing protein	-
HV236_RS18850	3906529..3907011	-	483	WP_032250314.1	DNA repair protein RadC	-
HV236_RS18855	3907020..3907481	-	462	WP_016239686.1	antirestriction protein	-
HV236_RS18860	3907495..3907686	-	192	Protein_3680	DUF905 family protein	-
HV236_RS18865	3907959..3908858	-	900	WP_111975973.1	transcriptional regulator GcvA	-
HV236_RS18870	3908977..3909309	+	333	WP_111975974.1	gamma-glutamylcyclotransferase	-
HV236_RS18875	3909422..3910309	+	888	WP_163415869.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	3803803..3908858	105055

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13020.04 Da
• Isoelectric Point: 9.9961

>T163975 WP_016243829.1 NZ_CP056707:c3905942-3905601 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T163975 NZ_CP072784:c849666-849514 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12020.72 Da
• Isoelectric Point: 7.6693

>AT163975 WP_150320518.1 NZ_CP056707:c3906280-3905963 [Enterobacter hormaechei]
MCNITWGLQRNITPRLGARLVQEGNRMHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEMNPRNAHCVTLFH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT163975 NZ_CP072784:849715-849772 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: A0A411GL63