Detailed information of TA system TA163927

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45232..45486
• Replicon: plasmid pRHBSTW-00605_2
• Accession: NZ_CP056698
• Organism: Escherichia marmotae strain RHBSTW-00605

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HV246_RS23560
• Protein ID: WP_001312851.1
• Coordinates: 45232..45381 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	45428..45486 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV246_RS23525	40862..41149	-	288	WP_024195838.1	helix-turn-helix transcriptional regulator	-
HV246_RS23530	41149..41460	-	312	WP_000483534.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV246_RS23535	42434..43291	-	858	WP_021555340.1	incFII family plasmid replication initiator RepA	-
HV246_RS23540	43284..43358	-	75	WP_011387702.1	RepA leader peptide Tap	-
HV246_RS23545	43593..43853	-	261	WP_000078108.1	replication regulatory protein RepA	-
HV246_RS23550	44138..44620	-	483	WP_001558270.1	GNAT family N-acetyltransferase	-
HV246_RS23555	44611..44901	-	291	WP_001558271.1	DUF1778 domain-containing protein	-
HV246_RS23560	45232..45381	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	45428..45486	+	59	NuclAT_1	-	Antitoxin
-	45428..45486	+	59	NuclAT_1	-	Antitoxin
-	45428..45486	+	59	NuclAT_1	-	Antitoxin
-	45428..45486	+	59	NuclAT_1	-	Antitoxin
HV246_RS23565	46429..47028	-	600	Protein_49	hypothetical protein	-
HV246_RS23570	47411..47611	-	201	WP_001558275.1	hypothetical protein	-
HV246_RS23575	47744..48304	-	561	WP_001558276.1	fertility inhibition protein FinO	-
HV246_RS23580	48378..49136	-	759	WP_021555343.1	type-F conjugative transfer system pilin acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..127911	127911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T163927 WP_001312851.1 NZ_CP056698:c45381-45232 [Escherichia marmotae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T163927 NZ_CP072765:c2206907-2206611 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT163927 NZ_CP056698:45428-45486 [Escherichia marmotae]
AGATACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin