Detailed information of TA system TA163725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 78922..79785
• Replicon: plasmid pRHBSTW-00370_4
• Accession: NZ_CP056576
• Organism: Citrobacter freundii strain RHBSTW-00370

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A7W3E442
• Locus tag: HV178_RS27410
• Protein ID: WP_044702672.1
• Coordinates: 78922..79416 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A5B0SRB7
• Locus tag: HV178_RS27415
• Protein ID: WP_044702675.1
• Coordinates: 79441..79785 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV178_RS27380	73948..74358	-	411	WP_181553766.1	DUF1380 family protein	-
HV178_RS27385	74979..75758	-	780	WP_181553767.1	hypothetical protein	-
HV178_RS27390	75918..76766	-	849	WP_181553768.1	DUF4942 domain-containing protein	-
HV178_RS27395	76884..77099	-	216	WP_044702680.1	hypothetical protein	-
HV178_RS27400	77168..77848	-	681	WP_044702678.1	hypothetical protein	-
HV178_RS27405	77946..78194	-	249	WP_044702684.1	hypothetical protein	-
HV178_RS27410	78922..79416	-	495	WP_044702672.1	type II toxin-antitoxin system YhaV family toxin	Toxin
HV178_RS27415	79441..79785	-	345	WP_044702675.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
HV178_RS27420	80222..80452	-	231	WP_044702671.1	hypothetical protein	-
HV178_RS27425	80443..80706	-	264	WP_044702670.1	DUF1380 family protein	-
HV178_RS27430	80813..81094	-	282	WP_044702669.1	hypothetical protein	-
HV178_RS27435	81242..81760	-	519	WP_181553769.1	hypothetical protein	-
HV178_RS27440	81750..82124	-	375	WP_044702665.1	hypothetical protein	-
HV178_RS27445	82134..82463	-	330	WP_044702664.1	hypothetical protein	-
HV178_RS27450	82477..82827	-	351	WP_181553770.1	hypothetical protein	-
HV178_RS27455	82855..83286	-	432	WP_049002914.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..101715	101715

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (11-147)

Antitoxin

• PrlF antitoxin (16-113)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 19076.90 Da
• Isoelectric Point: 8.2557

>T163725 WP_044702672.1 NZ_CP056576:c79416-78922 [Citrobacter freundii]
MEYLEINGWKICFHKCFLDQITELAKKVAELKAAKPSEYHTKKETKLLRAIERVIEEWIASDPLNPQYRQGETLGDEYKH
WFRVKFLQQFRLFFRCSQEHKTIIIGWVNDFGTLRAYGSKTDAYKVFAAMLDAGNPPDDWDKLLNAATKATATASIPDFL
TRQP

Nucleotide

• Length: 495 bp

>T163725 NZ_CP072762:c695234-694842 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12537.26 Da
• Isoelectric Point: 4.6787

>AT163725 WP_044702675.1 NZ_CP056576:c79785-79441 [Citrobacter freundii]
MATQILNSADVSLRAESRLTERSQTTIPAAIRDALHLKPGESIHYTLLAGGKVIMSRKDEEQDDPVVSQFLAFLEKDMKK
NPQNIAPVPAFFWESIKSLTAGVEVDLDAPLTDS

Nucleotide

• Length: 345 bp

>AT163725 NZ_CP072762:c695488-695231 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3E442

Antitoxin

• AlphaFold DB: A0A5B0SRB7