Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 136877..137402 Replicon plasmid pRHBSTW-00370_3
Accession NZ_CP056575
Organism Citrobacter freundii strain RHBSTW-00370

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag HV178_RS26880 Protein ID WP_008322213.1
Coordinates 137097..137402 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag HV178_RS26875 Protein ID WP_032934863.1
Coordinates 136877..137095 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV178_RS26845 131925..132341 - 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin -
HV178_RS26850 132338..132568 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HV178_RS26855 132821..133843 - 1023 WP_008322227.1 helicase UvrD -
HV178_RS26860 133828..135390 - 1563 WP_008322224.1 AAA family ATPase -
HV178_RS26865 135464..135880 - 417 WP_008322222.1 type II toxin-antitoxin system VapC family toxin -
HV178_RS26870 135877..136107 - 231 WP_008322219.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HV178_RS26875 136877..137095 + 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV178_RS26880 137097..137402 + 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
HV178_RS26885 137404..137718 + 315 WP_008322211.1 hypothetical protein -
HV178_RS26890 137708..138499 + 792 WP_008322208.1 site-specific integrase -
HV178_RS26895 138698..140065 - 1368 WP_008322207.1 GntP family transporter -
HV178_RS26900 140151..140927 - 777 WP_008322204.1 HPr family phosphocarrier protein -
HV178_RS26905 140932..141570 - 639 WP_008322203.1 aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..148724 148724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T163724 WP_008322213.1 NZ_CP056575:137097-137402 [Citrobacter freundii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T163724 NZ_CP072762:677925-678332 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT163724 WP_032934863.1 NZ_CP056575:136877-137095 [Citrobacter freundii]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT163724 NZ_CP072762:677713-677928 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References