Detailed information of TA system TA163720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 217364..218265
• Replicon: plasmid pRHBSTW-00370_2
• Accession: NZ_CP056574
• Organism: Citrobacter freundii strain RHBSTW-00370

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: HV178_RS25930
• Protein ID: WP_008786589.1
• Coordinates: 217364..217822 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HV178_RS25935
• Protein ID: WP_008786588.1
• Coordinates: 217819..218265 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV178_RS25885	213090..213332	+	243	WP_007372291.1	hypothetical protein	-
HV178_RS25890	213343..213741	+	399	WP_007372292.1	hypothetical protein	-
HV178_RS25895	213825..214151	+	327	WP_007372293.1	hypothetical protein	-
HV178_RS25900	214514..214852	-	339	WP_007372294.1	hypothetical protein	-
HV178_RS25905	215090..215533	+	444	WP_007372295.1	hypothetical protein	-
HV178_RS25910	215652..215993	+	342	WP_007372296.1	hypothetical protein	-
HV178_RS25915	216152..216706	+	555	WP_181553746.1	hypothetical protein	-
HV178_RS25920	216756..217022	+	267	WP_007372298.1	hypothetical protein	-
HV178_RS25925	217090..217320	-	231	WP_007372299.1	hypothetical protein	-
HV178_RS25930	217364..217822	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
HV178_RS25935	217819..218265	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
HV178_RS25940	218414..218731	+	318	WP_016241555.1	hypothetical protein	-
HV178_RS25945	218773..219615	+	843	WP_007372303.1	hypothetical protein	-
HV178_RS25950	219587..219865	+	279	WP_007372304.1	hypothetical protein	-
HV178_RS25955	219925..220368	+	444	WP_007372305.1	hypothetical protein	-
HV178_RS25960	220655..220819	+	165	WP_007372306.1	hypothetical protein	-
HV178_RS25965	221387..222229	+	843	WP_007372307.1	HNH endonuclease	-
HV178_RS25970	222651..222902	+	252	WP_024196080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..254427	254427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T163720 WP_008786589.1 NZ_CP056574:c217822-217364 [Citrobacter freundii]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T163720 NZ_CP072762:363476-363778 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT163720 WP_008786588.1 NZ_CP056574:c218265-217819 [Citrobacter freundii]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT163720 NZ_CP072762:363826-364047 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Toxin

• AlphaFold DB: S1EZ83