Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3774858..3775478 Replicon chromosome
Accession NZ_CP056573
Organism Citrobacter freundii strain RHBSTW-00370

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV178_RS18375 Protein ID WP_002892050.1
Coordinates 3775260..3775478 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV178_RS18370 Protein ID WP_003021733.1
Coordinates 3774858..3775232 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV178_RS18360 3770004..3771197 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV178_RS18365 3771220..3774369 + 3150 WP_121566979.1 efflux RND transporter permease AcrB -
HV178_RS18370 3774858..3775232 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV178_RS18375 3775260..3775478 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV178_RS18380 3775785..3776336 + 552 WP_032936967.1 maltose O-acetyltransferase -
HV178_RS18385 3776453..3776923 + 471 WP_003021724.1 YlaC family protein -
HV178_RS18390 3777002..3777142 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV178_RS18395 3777144..3777404 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
HV178_RS18400 3777593..3779146 + 1554 WP_101739942.1 EAL domain-containing protein -
HV178_RS18405 3779198..3779551 - 354 WP_003831033.1 DUF1428 family protein -
HV178_RS18410 3779616..3780245 - 630 WP_003021707.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163716 WP_002892050.1 NZ_CP056573:3775260-3775478 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163716 NZ_CP072761:c3848137-3847745 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163716 WP_003021733.1 NZ_CP056573:3774858-3775232 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163716 NZ_CP072761:c3848412-3848137 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References