Detailed information of TA system TA163714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2469474..2470113
• Replicon: chromosome
• Accession: NZ_CP056573
• Organism: Citrobacter freundii strain RHBSTW-00370

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: HV178_RS11855
• Protein ID: WP_003020221.1
• Coordinates: 2469474..2469650 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV178_RS11860
• Protein ID: WP_123924900.1
• Coordinates: 2469697..2470113 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV178_RS11835	2466252..2466482	-	231	WP_003020233.1	DUF2554 family protein	-
HV178_RS11840	2466672..2466797	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV178_RS11845	2466797..2467807	-	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
HV178_RS11850	2467807..2469210	-	1404	WP_003843730.1	cytochrome ubiquinol oxidase subunit I	-
HV178_RS11855	2469474..2469650	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV178_RS11860	2469697..2470113	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV178_RS11865	2470206..2471615	+	1410	WP_044702348.1	PLP-dependent aminotransferase family protein	-
HV178_RS11870	2471944..2473089	+	1146	WP_181553732.1	ABC transporter substrate-binding protein	-
HV178_RS11875	2473106..2474122	+	1017	WP_003020210.1	ABC transporter ATP-binding protein	-
HV178_RS11880	2474123..2475067	+	945	WP_003843727.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T163714 WP_003020221.1 NZ_CP056573:2469474-2469650 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T163714 NZ_CP072761:c3756563-3756135 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT163714 WP_123924900.1 NZ_CP056573:2469697-2470113 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT163714 NZ_CP072761:c3756809-3756567 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36