Detailed information of TA system TA163705

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1196598..1197277
• Replicon: chromosome
• Accession: NZ_CP056573
• Organism: Citrobacter freundii strain RHBSTW-00370

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7D6YND6
• Locus tag: HV178_RS05730
• Protein ID: WP_032642920.1
• Coordinates: 1196598..1196894 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A9K1QZR3
• Locus tag: HV178_RS05735
• Protein ID: WP_032642918.1
• Coordinates: 1196954..1197277 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV178_RS05705	1191705..1193609	+	1905	WP_048232635.1	5-dehydro-2-deoxygluconokinase	-
HV178_RS05710	1193624..1194514	+	891	WP_048232633.1	myo-inosose-2 dehydratase	-
HV178_RS05715	1194524..1195342	+	819	WP_048232631.1	5-deoxy-glucuronate isomerase	-
HV178_RS05720	1195557..1195922	-	366	Protein_1118	autotransporter outer membrane beta-barrel domain-containing protein	-
HV178_RS05725	1195930..1196091	-	162	Protein_1119	integrase arm-type DNA-binding domain-containing protein	-
HV178_RS05730	1196598..1196894	-	297	WP_032642920.1	TA system toxin CbtA family protein	Toxin
HV178_RS05735	1196954..1197277	-	324	WP_032642918.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV178_RS05740	1197299..1197520	-	222	WP_032642916.1	DUF987 domain-containing protein	-
HV178_RS05745	1197529..1197831	-	303	Protein_1123	hypothetical protein	-
HV178_RS05750	1197913..1198317	+	405	WP_000839182.1	IS66 family insertion sequence hypothetical protein	-
HV178_RS05755	1198314..1198661	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
HV178_RS05760	1198710..1200248	+	1539	WP_008786790.1	IS66 family transposase	-
HV178_RS05765	1200282..1200461	-	180	WP_181553744.1	hypothetical protein	-
HV178_RS05770	1200494..1201315	-	822	WP_181553735.1	DUF945 domain-containing protein	-
HV178_RS05775	1201536..1201976	-	441	WP_135695985.1	NirD/YgiW/YdeI family stress tolerance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	galE / acrB	1100823..1233823	133000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (4-97)

Antitoxin

• Antitoxin CbeA (4-102)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11228.87 Da
• Isoelectric Point: 6.0673

>T163705 WP_032642920.1 NZ_CP056573:c1196894-1196598 [Citrobacter freundii]
VPVSSRLSPVMVWQQLLTYLLEHHYGLTLNDTPFHDDTAIEEHIDAGITLADAVNFLVEKYELARIDRKGFTWREQPPFI
TATDILRAKKNIGLLPRS

Nucleotide

• Length: 297 bp

>T163705 NZ_CP072761:c3025356-3025033 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12082.82 Da
• Isoelectric Point: 6.6250

>AT163705 WP_032642918.1 NZ_CP056573:c1197277-1196954 [Citrobacter freundii]
MQNSWGLKRDISPCFGARLVQEGCRLHFLADRAGISGTFNEEEALRLDQAFPLVMKQLELMLTTGELNPRHQHCVTLYHN
GLTCEADTLGSHGYVYIVIYPQAFATR

Nucleotide

• Length: 324 bp

>AT163705 NZ_CP072761:c3025681-3025436 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Structures

Toxin

• AlphaFold DB: A0A7D6YND6