Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 260525..261156 Replicon chromosome
Accession NZ_CP056573
Organism Citrobacter freundii strain RHBSTW-00370

Toxin (Protein)


Gene name hicA Uniprot ID A0A0J1MPQ6
Locus tag HV178_RS01210 Protein ID WP_003837806.1
Coordinates 260525..260800 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0J1MMS0
Locus tag HV178_RS01215 Protein ID WP_003837808.1
Coordinates 260797..261156 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV178_RS01200 256576..259323 + 2748 WP_048218800.1 ribosome-associated ATPase/putative transporter RbbA -
HV178_RS01205 259323..260447 + 1125 WP_048232965.1 ABC transporter permease -
HV178_RS01210 260525..260800 + 276 WP_003837806.1 type II toxin-antitoxin system HicA family toxin Toxin
HV178_RS01215 260797..261156 + 360 WP_003837808.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HV178_RS01220 261269..261670 - 402 WP_003023387.1 nickel-responsive transcriptional regulator NikR -
HV178_RS01225 261720..262298 - 579 WP_003023390.1 4'-phosphopantetheinyl transferase AcpT -
HV178_RS01230 262361..263410 - 1050 WP_003023393.1 AI-2E family transporter -
HV178_RS01235 263544..264761 + 1218 WP_016151126.1 MFS transporter -
HV178_RS01240 264765..265322 - 558 WP_003837816.1 DcrB family lipoprotein -
HV178_RS01245 265395..266060 - 666 WP_003023401.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10210.88 Da        Isoelectric Point: 10.7228

>T163703 WP_003837806.1 NZ_CP056573:260525-260800 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Download         Length: 276 bp

>T163703 NZ_CP072761:c2918569-2918174 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13198.98 Da        Isoelectric Point: 4.5462

>AT163703 WP_003837808.1 NZ_CP056573:260797-261156 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Download         Length: 360 bp

>AT163703 NZ_CP072761:c2918811-2918566 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MPQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MMS0

References