Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2298877..2299439 Replicon chromosome
Accession NZ_CP056473
Organism Citrobacter freundii strain RHBSTW-00477

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag HV211_RS10730 Protein ID WP_181632218.1
Coordinates 2299161..2299439 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HV211_RS10725 Protein ID WP_096757032.1
Coordinates 2298877..2299161 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV211_RS10710 2294181..2297228 + 3048 WP_117342799.1 formate dehydrogenase-N subunit alpha -
HV211_RS10715 2297242..2298123 + 882 WP_181632217.1 formate dehydrogenase N subunit beta -
HV211_RS10720 2298116..2298772 + 657 WP_096757031.1 formate dehydrogenase-N subunit gamma -
HV211_RS10725 2298877..2299161 - 285 WP_096757032.1 HigA family addiction module antidote protein Antitoxin
HV211_RS10730 2299161..2299439 - 279 WP_181632218.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HV211_RS10735 2299627..2301342 - 1716 WP_181632219.1 ATP-binding cassette domain-containing protein -
HV211_RS10740 2301653..2303350 - 1698 WP_181632220.1 malate dehydrogenase -
HV211_RS10745 2303530..2303670 - 141 WP_096757036.1 stationary-phase-induced ribosome-associated protein -
HV211_RS10750 2303899..2304330 + 432 WP_096757037.1 OsmC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10528.00 Da        Isoelectric Point: 9.5320

>T163568 WP_181632218.1 NZ_CP056473:c2299439-2299161 [Citrobacter freundii]
MIMNFRHKGLRDLFLQGRTSGVTAKHVRRLRHRLAVIDAASHVTDINMPGYKLHALTGDRDGVLSISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T163568 NZ_CP072686:2633207-2633485 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10327.05 Da        Isoelectric Point: 8.8094

>AT163568 WP_096757032.1 NZ_CP056473:c2299161-2298877 [Citrobacter freundii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNAWSLAEAQ
KSVDISRLRKLVAQ

Download         Length: 285 bp

>AT163568 NZ_CP072686:2633485-2633769 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References