Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 57538..58274 Replicon plasmid pRHBSTW-00610_2
Accession NZ_CP056386
Organism Klebsiella pneumoniae strain RHBSTW-00610

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HV247_RS26210 Protein ID WP_003026803.1
Coordinates 57792..58274 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV247_RS26205 Protein ID WP_003026799.1
Coordinates 57538..57804 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV247_RS26185 54222..54530 - 309 WP_017896554.1 hypothetical protein -
HV247_RS26195 55942..56922 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
HV247_RS26200 56991..57293 + 303 Protein_62 cell envelope integrity protein TolA -
HV247_RS26205 57538..57804 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV247_RS26210 57792..58274 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HV247_RS26215 58475..59878 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HV247_RS26220 59907..60539 - 633 WP_001567369.1 hypothetical protein -
HV247_RS26225 61063..62067 - 1005 WP_000427620.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..252882 252882
- inside IScluster/Tn - - 54637..69388 14751


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T163367 WP_003026803.1 NZ_CP056386:57792-58274 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T163367 NZ_CP072554:c5007-4717 [Acinetobacter lwoffii]
ATGACACTGACGATTAGATATAAGAAACAATTTAAAAAAGATTTTAAACGGGTAAGTTCAAATCCAAAGCTTAAGAAAGG
TCTTTTAATTTTGAAAGATCTTATTGATGACCATTTGATAAAAACAGGGACTGTTCCAGATGAATATTTACCTCATCCTT
TGATTGGAGAATGGAGGCCTCACATGGAATGTCATGTCTTACCTGATTTATTGTTAATCTGGGATGTGGACAAAGAAAAT
AATGAGCTGATCCTTGCTAGGATAGGATCTCATTCTGAACTTTTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT163367 WP_003026799.1 NZ_CP056386:57538-57804 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT163367 NZ_CP072554:c5266-4991 [Acinetobacter lwoffii]
ATGACTACTTTGGCAAAAAAAGATGAGTATGTACGTGCTAGGGTTCCAAATGAATTAAAAGTTCAAGCGGAGCGAATTCT
AAAAGAAGTAGGTTTAAATACTACGGATGCTATTCGCTTATTATTAACGCAAGTAGTAAATCGTGGTGGATTTCCTTTAG
AGTTAAGAACCCCTAACCGTACAACAATTGAGGCTATGAACTCTGCTGCAGAGCCTAAAAAATTTAGTTCTGCTCAGGAA
TTGTTTACGGATTTAGAAGATGACACTGACGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References