Detailed information of TA system TA163261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3920868..3921465
• Replicon: chromosome
• Accession: NZ_CP056307
• Organism: Klebsiella pneumoniae strain RHBSTW-00832

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: HV292_RS19425
• Protein ID: WP_004893639.1
• Coordinates: 3921148..3921465 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: HV292_RS19420
• Protein ID: WP_004142561.1
• Coordinates: 3920868..3921155 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV292_RS19390	3916948..3917196	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
HV292_RS19395	3917214..3917555	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
HV292_RS19400	3917586..3918701	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
HV292_RS19405	3918881..3919462	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
HV292_RS19410	3919462..3919830	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
HV292_RS19415	3919950..3920603	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
HV292_RS19420	3920868..3921155	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
HV292_RS19425	3921148..3921465	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV292_RS19430	3921650..3922693	-	1044	WP_009308098.1	DUF2157 domain-containing protein	-
HV292_RS19435	3923359..3924225	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
HV292_RS19440	3924334..3925761	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T163261 WP_004893639.1 NZ_CP056307:c3921465-3921148 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T163261 NZ_CP072492:732501-732986 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT163261 WP_004142561.1 NZ_CP056307:c3921155-3920868 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT163261 NZ_CP072492:732211-732504 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1