Detailed information of TA system TA162863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2406894..2407532
• Replicon: chromosome
• Accession: NZ_CP056165
• Organism: Escherichia marmotae strain RHBSTW-00777

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: HV276_RS11460
• Protein ID: WP_000813795.1
• Coordinates: 2407356..2407532 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV276_RS11455
• Protein ID: WP_077627724.1
• Coordinates: 2406894..2407310 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV276_RS11435	2402049..2402990	-	942	WP_181237914.1	ABC transporter permease	-
HV276_RS11440	2402991..2404004	-	1014	WP_016248715.1	ABC transporter ATP-binding protein	-
HV276_RS11445	2404022..2405167	-	1146	WP_000047463.1	ABC transporter substrate-binding protein	-
HV276_RS11450	2405406..2406815	-	1410	WP_181502684.1	PLP-dependent aminotransferase family protein	-
HV276_RS11455	2406894..2407310	-	417	WP_077627724.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HV276_RS11460	2407356..2407532	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HV276_RS11465	2407754..2407984	+	231	WP_181502686.1	DUF2554 family protein	-
HV276_RS11470	2408078..2410039	-	1962	WP_181503346.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HV276_RS11475	2410112..2410648	-	537	WP_000429073.1	XRE family transcriptional regulator	-
HV276_RS11480	2410701..2411912	+	1212	WP_071990339.1	benzoate/H(+) symporter BenE family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T162863 WP_000813795.1 NZ_CP056165:c2407532-2407356 [Escherichia marmotae]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T162863 NZ_CP072146:c1519691-1519389 [Rhizobium sp. K1/93]
TTGCCAGTGAAGACGCTACCTTTCGATGCTGCAGATCATTTGCAGAGCGGCCAATCCCAGCTTGAGTTGCTGAGCGATGC
TTTTAATTCCGGGAGCGCATCCTATGTCGCGGTGGCGTTGGGCGTCATTGCGCGCGCCAAAGGCATGACGGAAGTATCGA
AAACGGCAGGCATCACCCGCGAAGCGCTATACAAAGCTCTCAGCGCCGACGGCGATCCGAAGCTGAGCACGCTGCTTGGC
GTGCTCAACGCTCTCGACCTCCAGCTGAAATTGGCCCCTGGCAACAAAAAAGCCCCGGACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15250.62 Da
• Isoelectric Point: 4.5908

>AT162863 WP_077627724.1 NZ_CP056165:c2407310-2406894 [Escherichia marmotae]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNNNDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT162863 NZ_CP072146:c1519985-1519692 [Rhizobium sp. K1/93]
ATGCCCGAAGTTCGGCAGACGATCTTGTTCAGGCGTTGGTTCGATGGCCTGAAAGACAAGCGTGCGGTTGAGCGGATCAC
CCAGCGTATCGTTCGACTGCAAGCCGGTTTGTTTGGAGACATAAAATTCTTCGATGGCATCGGCGAGTTGCGCGTCGATC
ATGGGCCTGGGTATCGGGTTTATTTTGTGCAGCGCGGATCTGTCCTGGTCATTTTACTGTGGGGCGGCGACAAGAGTTCT
CAAACCCGCGATATACGGAAAGCCAAGGCCATGGCGAATGATTTGGAGATTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1