Detailed information of TA system TA162817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 35786..36429
• Replicon: plasmid pRHBSTW-00844_3
• Accession: NZ_CP056157
• Organism: Enterobacter hormaechei strain RHBSTW-00844

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A5P6KDG5
• Locus tag: HV295_RS23170
• Protein ID: WP_021312768.1
• Coordinates: 35786..36202 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A5P6KGW8
• Locus tag: HV295_RS23175
• Protein ID: WP_021312767.1
• Coordinates: 36199..36429 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV295_RS23140	31724..32509	-	786	WP_003032097.1	ABC transporter substrate-binding protein	-
HV295_RS23145	32736..33500	+	765	WP_032192077.1	GntR family transcriptional regulator	-
HV295_RS23150	33601..34353	-	753	Protein_38	VOC family protein	-
HV295_RS23155	34350..34679	-	330	Protein_39	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
HV295_RS23160	34745..35449	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HV295_RS23165	35455..35688	+	234	WP_089617563.1	helix-turn-helix domain-containing protein	-
HV295_RS23170	35786..36202	-	417	WP_021312768.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV295_RS23175	36199..36429	-	231	WP_021312767.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV295_RS23180	37056..37274	+	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	-
HV295_RS23185	37276..37581	+	306	WP_045616569.1	type II toxin-antitoxin system toxin CcdB	-
HV295_RS23190	37751..38728	+	978	Protein_46	hypothetical protein	-
HV295_RS23195	38964..39758	+	795	WP_016240356.1	site-specific integrase	-
HV295_RS23200	40227..40406	-	180	WP_006788217.1	hypothetical protein	-
HV295_RS23205	40526..40636	-	111	Protein_49	cobyrinic acid ac-diamide synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..125868	125868
	-	flank	IS/Tn	-	-	34745..35449	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15175.60 Da
• Isoelectric Point: 7.2452

>T162817 WP_021312768.1 NZ_CP056157:c36202-35786 [Enterobacter hormaechei]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T162817 NZ_CP072119:4820180-4820398 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8722.81 Da
• Isoelectric Point: 4.4692

>AT162817 WP_021312767.1 NZ_CP056157:c36429-36199 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT162817 NZ_CP072119:4819779-4820153 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A5P6KDG5

Antitoxin

• AlphaFold DB: A0A5P6KGW8