Detailed information of TA system TA162814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4308612..4309228
• Replicon: chromosome
• Accession: NZ_CP056155
• Organism: Enterobacter hormaechei strain RHBSTW-00844

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV295_RS20700
• Protein ID: WP_077266491.1
• Coordinates: 4308612..4308983 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: HV295_RS20705
• Protein ID: WP_015569912.1
• Coordinates: 4308986..4309228 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV295_RS20685	4306112..4307014	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
HV295_RS20690	4307011..4307646	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
HV295_RS20695	4307643..4308572	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
HV295_RS20700	4308612..4308983	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV295_RS20705	4308986..4309228	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV295_RS20710	4309427..4310347	+	921	WP_047051644.1	alpha/beta hydrolase	-
HV295_RS20715	4310356..4311297	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
HV295_RS20720	4311342..4311779	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV295_RS20725	4311776..4312657	-	882	WP_003861949.1	virulence factor BrkB family protein	-
HV295_RS20730	4312651..4313250	-	600	WP_032649898.1	glucose-1-phosphatase	-
HV295_RS20735	4313369..4314169	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T162814 WP_077266491.1 NZ_CP056155:c4308983-4308612 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T162814 NZ_CP072119:c1555581-1555207 [Klebsiella michiganensis]
ATGCAAAAACAATCCCTGTCCCCTCATAGGGCGGCCTCGTCACGTCTGTCATCAGTCGAAATCTGGCGAAAATTATTGAA
GTATCTGCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAGCACATTG
ATGCGGGAATCTCACTGTGTTACGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCAGGAAGGCTACCGGGCTGATGACACGCAA
GGGATATAAAACCGTTACTGACATCTCAGGTGGCAGATCCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT162814 WP_015569912.1 NZ_CP056155:c4309228-4308986 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT162814 NZ_CP072119:c1555990-1555631 [Klebsiella michiganensis]
ATGCAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACACCGTGTTT
TGGTGCACGACTGGTACATGAGGGTAACCACCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAACAGATGGAGCTCATGCTGACCAGTAGCGAACTCACG
CCCCACATTCAGCGCTGCATTACGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATTT
GTACATCGTAATTTATCCGGCGTCTGCAACAACCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3