Detailed information of TA system TA162811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2662873..2663533
• Replicon: chromosome
• Accession: NZ_CP056155
• Organism: Enterobacter hormaechei strain RHBSTW-00844

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV295_RS13030
• Protein ID: WP_047053529.1
• Coordinates: 2663180..2663533 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0Q4Z7
• Locus tag: HV295_RS13025
• Protein ID: WP_017383313.1
• Coordinates: 2662873..2663175 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV295_RS13010	2659043..2660368	+	1326	WP_047053533.1	SidA/IucD/PvdA family monooxygenase	-
HV295_RS13015	2660395..2662584	+	2190	WP_047053531.1	TonB-dependent siderophore receptor	-
HV295_RS13025	2662873..2663175	-	303	WP_017383313.1	helix-turn-helix transcriptional regulator	Antitoxin
HV295_RS13030	2663180..2663533	-	354	WP_047053529.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV295_RS13035	2663724..2664674	-	951	WP_003859532.1	HTH-type transcriptional regulator Cbl	-
HV295_RS13040	2664771..2665688	-	918	WP_003859531.1	nitrogen assimilation transcriptional regulator NAC	-
HV295_RS13050	2666177..2667100	-	924	WP_047053526.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13522.40 Da
• Isoelectric Point: 10.1729

>T162811 WP_047053529.1 NZ_CP056155:c2663533-2663180 [Enterobacter hormaechei]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFNPNRTGI
VLCAGNKIGNERRFYDEMLLVADREYTRWLNTLKERN

Nucleotide

• Length: 354 bp

>T162811 NZ_CP072119:753378-753719 [Klebsiella michiganensis]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCCGGGATTACCCTGGCCGATGCAGTCAACTTCCTGGTGGATAAGTACGAACTGGTTCGTATCGATCGCAGGGGGTTT
AACTGGCAGGAGCAATCCCCTTATCTTCGGGCGGTAGATATCCTGCGAGCACGACAAGCAACTGGCTTGTTGCGACGGAG
CCGTAATAACGCTGTATCGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11028.85 Da
• Isoelectric Point: 6.4727

>AT162811 WP_017383313.1 NZ_CP056155:c2663175-2662873 [Enterobacter hormaechei]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT162811 NZ_CP072119:753016-753357 [Klebsiella michiganensis]
ATGAAATCTACTGACTCAGAGAATGATTCATTTCTCAAATGGGGTCTGAATCGTAATGAGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTAACCGGGCCAGCATTACCGGTGAATTTATTAACACAGAATATT
TGAAGTTGGATGAAATATTTCCGCATTTTATCAGCCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCTCGCCAT
GCCCACTGCGTCACTCTATACCACAACGGTTTCACCTGCGAAGCCGATACACTTGGCAGTTGCGGCTACGTATACATCTC
CGTTTACCCTACTCAACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0Q4Z7