Detailed information of TA system TA162714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 64455..64980
• Replicon: plasmid pRHBSTW-00925_2
• Accession: NZ_CP056130
• Organism: Enterobacter roggenkampii strain RHBSTW-00925

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A7H9K2T5
• Locus tag: HV328_RS23405
• Protein ID: WP_045616569.1
• Coordinates: 64675..64980 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A8H9ZZC6
• Locus tag: HV328_RS23400
• Protein ID: WP_016240352.1
• Coordinates: 64455..64673 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV328_RS23385	59642..60217	-	576	WP_060415435.1	hypothetical protein	-
HV328_RS23390	60214..60450	-	237	WP_060415434.1	hypothetical protein	-
HV328_RS23395	60766..63885	+	3120	WP_127472145.1	hypothetical protein	-
HV328_RS23400	64455..64673	+	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV328_RS23405	64675..64980	+	306	WP_045616569.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV328_RS23410	65150..66127	+	978	Protein_65	hypothetical protein	-
HV328_RS23415	66363..67157	+	795	WP_181519365.1	site-specific integrase	-
HV328_RS23420	67415..68395	+	981	Protein_67	IS5 family transposase	-
HV328_RS23425	68537..69505	+	969	WP_077257567.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..118256	118256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11571.24 Da
• Isoelectric Point: 6.4650

>T162714 WP_045616569.1 NZ_CP056130:64675-64980 [Enterobacter roggenkampii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRDLYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T162714 NZ_CP071974:44585-44956 [Trueperella pecoris]
ATGATTGTGGATACGTCCGCCCTGGTCGCAGTGGCATATAGGGAGCCCGGGTACGAAAGGATCACCGACCTTCTTTTCGC
AAGGAACAGGTCAAAGATGTCGGTGGCCAGCGTCCTTGAGCTTCAACTCGTATTGCGATCTTCCGAATTGGCGGACGAAC
TCATCGAGATTTTCAATGTGGATCCGGTGGGGATTGATGTCGCCCAGCTACGCGTGGCGCGGCACGCGGCGCAGCGATTC
GGAAAGGGGACCGGCTCAAAGGCGGGGCTAAACTACGGCGACTGTTTCTCATACGCCCTCGCGATGACTGCCGGAGAGCC
TTTGTTGTTTGTGGGCGAGGATTTCACGCACACGGACGTCGTCGTTGCGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8321.25 Da
• Isoelectric Point: 4.5358

>AT162714 WP_016240352.1 NZ_CP056130:64455-64673 [Enterobacter roggenkampii]
MKQRITVTIDSDSYQLLKSADVNISGLVNTAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFAEENRDW

Nucleotide

• Length: 219 bp

>AT162714 NZ_CP071974:44346-44588 [Trueperella pecoris]
ATGCCAACCATGAACATCAAAGATGAAGACGTGTACAACGCAGCTCGCCAGCTTGCACAGTTGCGTGGAACGTCGATGAC
TGGTGCCGTGCGTTCTGCAGTGGAAGAGGCTCTGGATGCCGAGATCAAGCGGCGCGAACGCGGGCGCTTGCGCCTTGCCG
AACTCCAAGACCGCATCGCGCAGGCCGCCGAGCCCCGCTTAGACGAGTCTGACCTGTATGACGATAGGGGAATGCCCCGA
TGA

Structures

Toxin

• AlphaFold DB: A0A7H9K2T5