Detailed information of TA system TA162698

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 682551..683230
• Replicon: chromosome
• Accession: NZ_CP056129
• Organism: Enterobacter roggenkampii strain RHBSTW-00925

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A1Z3N0J5
• Locus tag: HV328_RS03305
• Protein ID: WP_045268641.1
• Coordinates: 682889..683230 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV328_RS03300
• Protein ID: WP_181519123.1
• Coordinates: 682551..682868 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV328_RS03265	677667..679322	-	1656	WP_181519320.1	AAA family ATPase	-
HV328_RS03270	679321..680528	+	1208	WP_116799391.1	IS3 family transposase	-
HV328_RS03275	680563..680676	-	114	WP_181519122.1	AAA family ATPase	-
HV328_RS03280	681065..681249	+	185	Protein_637	DUF905 family protein	-
HV328_RS03285	681351..681809	+	459	WP_045268638.1	antirestriction protein	-
HV328_RS03290	681825..682301	+	477	WP_058673375.1	RadC family protein	-
HV328_RS03295	682310..682531	+	222	WP_007893437.1	DUF987 domain-containing protein	-
HV328_RS03300	682551..682868	+	318	WP_181519123.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV328_RS03305	682889..683230	+	342	WP_045268641.1	TA system toxin CbtA family protein	Toxin
HV328_RS03315	683592..684098	+	507	WP_008503183.1	G/U mismatch-specific DNA glycosylase	-
HV328_RS03320	684188..686035	-	1848	WP_008503184.1	RNA polymerase sigma factor RpoD	-
HV328_RS03325	686190..687935	-	1746	WP_023294585.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	652718..684098	31380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13045.30 Da
• Isoelectric Point: 10.5786

>T162698 WP_045268641.1 NZ_CP056129:682889-683230 [Enterobacter roggenkampii]
MKTLPATTLRVVKPCLSPVAIWKMLLTRLLEQHYGLTLNDTPFSKERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRKNLVR

Nucleotide

• Length: 342 bp

>T162698 NZ_CP071955:c4029537-4029256 [Xanthomonas fragariae]
ATGATCAGGAGCTTTGTCGACAAGGAAGCCGAAAAGATCTGGATGGGTGAGCGCTCCCGCCGGTTGCCGGCCGACATCCA
GTCGGTCGCGCGCCGCAAGTTGCGCATGCTCAACGCTGCCGCGCACCTCGACGATCTGCGCATTCCGCCTGCCAACCGGC
TGGAAGCGTTGAAGGGCGAGCGGCGCGGGCAGTACAGCATCCGGATCAACGACCAGTGGCGTATCTGCTTCCGATGGATG
GAGGGCGATGTGGCCGAGGTCGAAATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11988.72 Da
• Isoelectric Point: 6.7379

>AT162698 WP_181519123.1 NZ_CP056129:682551-682868 [Enterobacter roggenkampii]
MTHIIWGLQRNITPRLGARLVQEGNRLHYLADRASITGMFSDVECLKLDDTFQHFIRQMESMLTTGELSPQHAHCVTLYH
NGFICEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT162698 NZ_CP071955:c4029237-4028947 [Xanthomonas fragariae]
ATGACAGTCCTACCCAACATCCACCCTGGTGAAATCCTGCTTGAGGAGTTCCTCGAGCCGATGGGTATCAGCCAGAACGC
ATTGGCGCGGGCCACCGGTGTGCCGCCACGCCGTATCAACGAAATCGTGTTGGGCAAGCGCGGCATCACCGCCGACACCG
CCGTGCGCCTGGCTGCAGCGCTGTGCACGACCGAGCGCTTCTGGCTAGGCCTGCAAGCCGATTACGAGCTGGAACAAGCG
CACCGCGCGCTGGGCGACCTGCCATCGCGGATCAAGCGGCTGGCGGCTTGA

Structures

Toxin

• AlphaFold DB: A0A1Z3N0J5