Detailed information of TA system TA162656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4094347..4095092
• Replicon: chromosome
• Accession: NZ_CP056118
• Organism: Enterobacter sp. DSM 30060

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HWQ15_RS19570
• Protein ID: WP_063148892.1
• Coordinates: 4094347..4094838 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: HWQ15_RS19575
• Protein ID: WP_003857131.1
• Coordinates: 4094826..4095092 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ15_RS19545	4090651..4090899	+	249	WP_080454250.1	DUF2384 domain-containing protein	-
HWQ15_RS19550	4091007..4092149	-	1143	WP_063148891.1	ATP-binding protein	-
HWQ15_RS19555	4092277..4092813	+	537	WP_063850159.1	hypothetical protein	-
HWQ15_RS19560	4093336..4093476	+	141	WP_162269090.1	hypothetical protein	-
HWQ15_RS19565	4093733..4094176	+	444	WP_139153383.1	hypothetical protein	-
HWQ15_RS19570	4094347..4094838	-	492	WP_063148892.1	GNAT family N-acetyltransferase	Toxin
HWQ15_RS19575	4094826..4095092	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
HWQ15_RS19580	4095156..4096085	-	930	WP_063148893.1	LysR family transcriptional regulator	-
HWQ15_RS19585	4096215..4097603	+	1389	WP_063148894.1	MFS transporter	-
HWQ15_RS19590	4097582..4098139	-	558	WP_023300131.1	OmpH family outer membrane protein	-
HWQ15_RS19595	4098260..4099396	-	1137	WP_063147317.1	type 1 fimbrial protein	-
HWQ15_RS19600	4099420..4099944	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4071814..4095092	23278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17829.52 Da
• Isoelectric Point: 9.6269

>T162656 WP_063148892.1 NZ_CP056118:c4094838-4094347 [Enterobacter sp. DSM 30060]
VGRVTPPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
QER

Nucleotide

• Length: 492 bp

>T162656 NZ_CP071947:c469361-469083 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT162656 WP_003857131.1 NZ_CP056118:c4095092-4094826 [Enterobacter sp. DSM 30060]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT162656 NZ_CP071947:c469071-468766 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9