Detailed information of TA system TA162516

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 760349..761190
• Replicon: chromosome
• Accession: NZ_CP056036
• Organism: Escherichia coli strain EC31

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: HUW39_RS03625
• Protein ID: WP_000854822.1
• Coordinates: 760807..761190 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HUW39_RS03620
• Protein ID: WP_048943268.1
• Coordinates: 760349..760717 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW39_RS03580 (755832)	755832..756371	+	540	WP_001104026.1	DUF4339 domain-containing protein	-
HUW39_RS03585 (756641)	756641..757054	+	414	WP_000789536.1	hypothetical protein	-
HUW39_RS03590 (757193)	757193..757371	-	179	Protein_714	hypothetical protein	-
HUW39_RS03595 (757465)	757465..758283	+	819	WP_048943269.1	DUF932 domain-containing protein	-
HUW39_RS03600 (758374)	758374..758859	+	486	WP_000214317.1	antirestriction protein	-
HUW39_RS03605 (758874)	758874..759350	+	477	WP_001384029.1	RadC family protein	-
HUW39_RS03610 (759419)	759419..759640	+	222	WP_000692353.1	DUF987 domain-containing protein	-
HUW39_RS03615 (759655)	759655..760299	+	645	WP_029396485.1	hypothetical protein	-
HUW39_RS03620 (760349)	760349..760717	+	369	WP_048943268.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUW39_RS03625 (760807)	760807..761190	+	384	WP_000854822.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
HUW39_RS03630 (761181)	761181..761669	+	489	WP_001177592.1	DUF5983 family protein	-
HUW39_RS03635 (761681)	761681..761878	+	198	WP_000839263.1	DUF957 domain-containing protein	-
HUW39_RS03640 (761975)	761975..762820	+	846	WP_001280435.1	DUF4942 domain-containing protein	-
HUW39_RS03650 (763119)	763119..763625	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
HUW39_RS03655 (763704)	763704..765545	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T162516 WP_000854822.1 NZ_CP056036:760807-761190 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T162516 NZ_CP071826:c1339507-1339229 [Burkholderia anthina]
ATGATCCAGTCGTTCAAATGCAAAGACACGCGGCAGCTTTTCGAAACGGGACGTTGCAGACAATTCGCCAACATTGCCGA
TGTCGCAATGCGTCGTCTTACGCAACTTGACAACGCGGCGACGCTGGACGCGATGAAATCACCGCCCGGCAACCGATTTG
AGGCACTGAAGGGCGATCGTGTTGGCCAGTACAGCATCCGAATCAACGGCCAATGGCGTGTGTGCTTTACATGGGGCGAT
GACGGGCCGGCCGATGTGGAAATCGCCGACTATCACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13645.39 Da
• Isoelectric Point: 6.4758

>AT162516 WP_048943268.1 NZ_CP056036:760349-760717 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT162516 NZ_CP071826:c1339216-1338902 [Burkholderia anthina]
ATGACCCGGTTCAAAAACGGTATGCGCCCGGTCCATCCGGGCGAAATTCTTCGTGAGGATTACCTCATCCCGCTCGACAT
GAGCGCGAACGCGTTGGCCGGCGCACTTGGCGTCACGGCCGGCCGCATCAACGATATCGTCCGTGAGCGGCGCGGCATCA
CCGCCGATACGGCGCTGCGCCTTGCCCGCTACTTTGGCGGCGATCCGCAAACGTGGCTGAACCTGCAAGCCACCTATGAC
CTGAAAATCGCTGAGCGCGACCACGGCCGCGAGATCCAGAAGCGCATCGAGCCCCGAGAATTCGCCCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1