Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1898621..1899233 Replicon chromosome
Accession NZ_CP056034
Organism Erwinia amylovora strain TS3128

Toxin (Protein)


Gene name hicA Uniprot ID D2TUP9
Locus tag HU055_RS08785 Protein ID WP_012906750.1
Coordinates 1898621..1898800 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID D4I356
Locus tag HU055_RS08790 Protein ID WP_004157632.1
Coordinates 1898823..1899233 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU055_RS08765 1894179..1895129 + 951 WP_004157624.1 DUF1471 domain-containing protein -
HU055_RS08770 1895337..1896728 + 1392 WP_004157625.1 amino acid permease -
HU055_RS08775 1896732..1897196 - 465 WP_004157626.1 hypothetical protein -
HU055_RS08780 1897373..1898101 + 729 WP_004157629.1 two-component system response regulator RstA -
HU055_RS08785 1898621..1898800 + 180 WP_012906750.1 type II toxin-antitoxin system HicA family toxin Toxin
HU055_RS08790 1898823..1899233 + 411 WP_004157632.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HU055_RS08795 1899289..1900289 + 1001 Protein_1708 acyltransferase -
HU055_RS08800 1901160..1901372 - 213 WP_004157635.1 hypothetical protein -
HU055_RS08805 1901630..1901803 - 174 WP_004157636.1 hypothetical protein -
HU055_RS08810 1901898..1902071 - 174 WP_013036045.1 hypothetical protein -
HU055_RS08815 1902082..1902354 - 273 WP_004157637.1 hypothetical protein -
HU055_RS08820 1902960..1903574 + 615 WP_004166850.1 acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1892459..1903574 11115


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6644.85 Da        Isoelectric Point: 10.9132

>T162508 WP_012906750.1 NZ_CP056034:1898621-1898800 [Erwinia amylovora]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Download         Length: 180 bp

>T162508 NZ_CP071823:c6804-6490 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGAGATTTACAACGCCTCCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGCCGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CATTTCTGCCGCAGGAATATCAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCGGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14829.76 Da        Isoelectric Point: 4.3623

>AT162508 WP_004157632.1 NZ_CP056034:1898823-1899233 [Erwinia amylovora]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESAREAIDAHIELLVEDGEAVPEATSVENWLADPDYVGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Download         Length: 411 bp

>AT162508 NZ_CP071823:c7070-6792 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AAGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCAGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6XDK4

Antitoxin

Source ID Structure
AlphaFold DB A0A830ZVY0

References