Detailed information of TA system TA162238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 784202..785039
• Replicon: chromosome
• Accession: NZ_CP055292
• Organism: Shigella sonnei strain SE6-1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HUX90_RS03835
• Protein ID: WP_000227784.1
• Coordinates: 784497..785039 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HUX90_RS03830
• Protein ID: WP_001297137.1
• Coordinates: 784202..784513 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUX90_RS03805	779222..780169	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
HUX90_RS03810	780191..782182	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HUX90_RS03815	782172..782786	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HUX90_RS03820	782786..783115	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HUX90_RS03825	783127..784017	+	891	WP_000971336.1	heme o synthase	-
HUX90_RS03830	784202..784513	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HUX90_RS03835	784497..785039	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HUX90_RS03840	785095..786030	-	936	WP_001297127.1	sel1 repeat family protein	-
HUX90_RS03845	786438..787802	+	1365	WP_001000978.1	MFS transporter	-
HUX90_RS03850	787930..788421	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HUX90_RS03855	788589..789500	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T162238 WP_000227784.1 NZ_CP055292:784497-785039 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T162238 NZ_CP071693:c4179334-4178843 [Salmonella sp. SAL-045]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT162238 WP_001297137.1 NZ_CP055292:784202-784513 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT162238 NZ_CP071693:c4179624-4179331 [Salmonella sp. SAL-045]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3