Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 120851..121503 Replicon plasmid unnamed1
Accession NZ_CP055175
Organism Klebsiella pneumoniae strain STEFF_19

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag HUZ47_RS26620 Protein ID WP_017901321.1
Coordinates 120851..121276 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag HUZ47_RS26625 Protein ID WP_001261275.1
Coordinates 121273..121503 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ47_RS26585 (HUZ47_26550) 116560..116676 + 117 Protein_123 transposase -
HUZ47_RS26590 (HUZ47_26555) 116801..116971 - 171 Protein_124 LysR family transcriptional regulator -
HUZ47_RS26595 116982..117209 + 228 Protein_125 IS3 family transposase -
HUZ47_RS26600 (HUZ47_26565) 117301..118857 - 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
HUZ47_RS26605 119044..119217 - 174 Protein_127 nuclease -
HUZ47_RS26610 (HUZ47_26570) 119270..119806 - 537 Protein_128 integrase core domain-containing protein -
HUZ47_RS26615 (HUZ47_26575) 119865..120833 - 969 WP_077254762.1 IS5 family transposase -
HUZ47_RS26620 (HUZ47_26580) 120851..121276 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ47_RS26625 (HUZ47_26585) 121273..121503 - 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ47_RS26630 (HUZ47_26590) 121756..124332 - 2577 WP_017901322.1 nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / tet(D) / aph(6)-Id / aph(3'')-Ib / catA1 / sul2 - 1..233421 233421
- flank IS/Tn - - 119865..120833 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T161758 WP_017901321.1 NZ_CP055175:c121276-120851 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T161758 NZ_CP071263:3570941-3571231 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT161758 WP_001261275.1 NZ_CP055175:c121503-121273 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT161758 NZ_CP071263:3570709-3570951 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGACTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References