Detailed information of TA system TA161750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3925612..3926209
• Replicon: chromosome
• Accession: NZ_CP055174
• Organism: Klebsiella pneumoniae strain STEFF_19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: HUZ47_RS18985
• Protein ID: WP_004893639.1
• Coordinates: 3925892..3926209 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: HUZ47_RS18980
• Protein ID: WP_004142561.1
• Coordinates: 3925612..3925899 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ47_RS18950 (HUZ47_18910)	3921692..3921940	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
HUZ47_RS18955 (HUZ47_18915)	3921958..3922299	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
HUZ47_RS18960 (HUZ47_18920)	3922330..3923445	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
HUZ47_RS18965 (HUZ47_18925)	3923625..3924206	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
HUZ47_RS18970 (HUZ47_18930)	3924206..3924574	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
HUZ47_RS18975 (HUZ47_18935)	3924694..3925347	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
HUZ47_RS18980 (HUZ47_18940)	3925612..3925899	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
HUZ47_RS18985 (HUZ47_18945)	3925892..3926209	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ47_RS18990 (HUZ47_18950)	3926394..3927437	-	1044	WP_004893645.1	DUF2157 domain-containing protein	-
HUZ47_RS18995 (HUZ47_18955)	3928103..3928969	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
HUZ47_RS19000 (HUZ47_18960)	3929078..3930505	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T161750 WP_004893639.1 NZ_CP055174:c3926209-3925892 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T161750 NZ_CP071263:2105618-2105944 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT161750 WP_004142561.1 NZ_CP055174:c3925899-3925612 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT161750 NZ_CP071263:2105160-2105528 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGTCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1