Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4396..4921 Replicon plasmid unnamed2
Accession NZ_CP055091
Organism Citrobacter portucalensis strain SWHIN_111

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag HUZ88_RS25040 Protein ID WP_008322213.1
Coordinates 4616..4921 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag HUZ88_RS25035 Protein ID WP_032934863.1
Coordinates 4396..4614 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ88_RS25025 (HUZ88_24955) 1042..2217 - 1176 WP_239606585.1 MFS transporter -
HUZ88_RS25030 (HUZ88_24960) 2775..3695 - 921 WP_113415726.1 hypothetical protein -
HUZ88_RS25035 (HUZ88_24965) 4396..4614 + 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HUZ88_RS25040 (HUZ88_24970) 4616..4921 + 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
HUZ88_RS25045 (HUZ88_24975) 4923..5237 + 315 WP_008322211.1 hypothetical protein -
HUZ88_RS25050 (HUZ88_24980) 5227..6018 + 792 WP_008322208.1 site-specific integrase -
HUZ88_RS25055 (HUZ88_24985) 6217..7584 - 1368 WP_008322207.1 GntP family transporter -
HUZ88_RS25060 (HUZ88_24990) 7670..8446 - 777 WP_008322204.1 HPr family phosphocarrier protein -
HUZ88_RS25065 (HUZ88_24995) 8451..9089 - 639 WP_008322203.1 aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..80984 80984


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T161442 WP_008322213.1 NZ_CP055091:4616-4921 [Citrobacter portucalensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T161442 NZ_CP071192:c4455865-4455611 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT161442 WP_032934863.1 NZ_CP055091:4396-4614 [Citrobacter portucalensis]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT161442 NZ_CP071192:c4455611-4455270 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGCGAGTT
TGTCGGGCTGAATGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCGCGG
TCTTTCTCGATGAGTGCCATAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCGCCG
GAACGCCATCAGGCGTTAGCCATTGCCGCACAGGCCACCGGGCAGTCGATTAATGAACTGCTGAATGAAGGTGTTGATCT
GGTTATCGAAAAGCATTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References