Detailed information of TA system TA161403

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 731534..732375
• Replicon: chromosome
• Accession: NZ_CP055087
• Organism: Klebsiella pneumoniae strain SWHIN_114

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: HUZ89_RS03675
• Protein ID: WP_000854822.1
• Coordinates: 731992..732375 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0CWL2
• Locus tag: HUZ89_RS03670
• Protein ID: WP_053271974.1
• Coordinates: 731534..731902 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ89_RS03650 (HUZ89_03650)	729279..730097	+	819	WP_001234702.1	DUF932 domain-containing protein	-
HUZ89_RS03655 (HUZ89_03655)	730188..730673	+	486	WP_000214317.1	antirestriction protein	-
HUZ89_RS03660 (HUZ89_03660)	730688..731164	+	477	WP_001186747.1	RadC family protein	-
HUZ89_RS03665 (HUZ89_03665)	731233..731454	+	222	WP_000692312.1	DUF987 domain-containing protein	-
HUZ89_RS03670 (HUZ89_03670)	731534..731902	+	369	WP_053271974.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ89_RS03675 (HUZ89_03675)	731992..732375	+	384	WP_000854822.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
HUZ89_RS03680 (HUZ89_03680)	732366..732854	+	489	WP_001177592.1	DUF5983 family protein	-
HUZ89_RS03685 (HUZ89_03685)	732821..733063	+	243	WP_166635458.1	DUF957 domain-containing protein	-
HUZ89_RS03690 (HUZ89_03690)	733160..734005	+	846	WP_001280435.1	DUF4942 domain-containing protein	-
HUZ89_RS03700 (HUZ89_03700)	734304..734810	+	507	WP_002917636.1	G/U mismatch-specific DNA glycosylase	-
HUZ89_RS03705 (HUZ89_03705)	734910..736751	-	1842	WP_004174339.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	aac(3)-IVa / aph(4)-Ia / floR / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrB4 / blaDHA-1	-	668947..734005	65058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T161403 WP_000854822.1 NZ_CP055087:731992-732375 [Klebsiella pneumoniae]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T161403 NZ_CP071176:1680384-1680728 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAGCGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAGTCTATATTCCATGAGTTCAAAC
ATGTTGTTTCCCATTCGGATTACATGGAGTTATATAAAATCCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCGGATCAT
CATATGTTTAAATGCCTTATTGAAAAACATGACGGGCAATTTAATTATTCTAATGTGATTACTCATTACAATTTAAAGAT
GGGACAAGAAACTTATTTAAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13679.41 Da
• Isoelectric Point: 6.4758

>AT161403 WP_053271974.1 NZ_CP055087:731534-731902 [Klebsiella pneumoniae]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT161403 NZ_CP071176:1680035-1680367 [Enterococcus faecalis]
ATGAACCTCTTGGAACGTATAAAAAAACTAGCAAAAGATAGAGATGTGTCTATTTATCAGCTTGAAGAAGAAATAAATAT
TGGACGAAATACTATTTATCAATGGAATAAGCGAACACCTTCTTCGGATAAACTTGAAGCTGTAGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTGGTGCTAAACCATCTGATGATTTAGATGAAGTA
TTAGACAATGTGATGAGTTTCGATGGTGAGCCTTTGGATGATCATGATCGGGAAGTTATTCGTGCTTATTTGAAAGGGAG
ATTCGGAAAATAA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1

Antitoxin

• AlphaFold DB: A0A1M0CWL2