Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5235835..5236460 Replicon chromosome
Accession NZ_CP055057
Organism Klebsiella pneumoniae strain SWHE4

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ67_RS25755 Protein ID WP_002882817.1
Coordinates 5235835..5236218 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ67_RS25760 Protein ID WP_004150355.1
Coordinates 5236218..5236460 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ67_RS25740 (HUZ67_25670) 5233201..5234103 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ67_RS25745 (HUZ67_25675) 5234100..5234735 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ67_RS25750 (HUZ67_25680) 5234732..5235661 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ67_RS25755 (HUZ67_25685) 5235835..5236218 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ67_RS25760 (HUZ67_25690) 5236218..5236460 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ67_RS25765 (HUZ67_25695) 5236665..5237582 + 918 WP_004178029.1 alpha/beta hydrolase -
HUZ67_RS25770 (HUZ67_25700) 5237596..5238537 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ67_RS25775 (HUZ67_25705) 5238582..5239019 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ67_RS25780 (HUZ67_25710) 5239016..5239876 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ67_RS25785 (HUZ67_25715) 5239870..5240469 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161294 WP_002882817.1 NZ_CP055057:c5236218-5235835 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161294 NZ_CP071127:3771037-3771294 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161294 WP_004150355.1 NZ_CP055057:c5236460-5236218 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161294 NZ_CP071127:3770765-3771040 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References