Detailed information of TA system TA161135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 184727..185454
• Replicon: plasmid unnamed1
• Accession: NZ_CP055034
• Organism: Klebsiella pneumoniae strain SWHEFF_62

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HUZ73_RS26580
• Protein ID: WP_011251285.1
• Coordinates: 185143..185454 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ73_RS26575
• Protein ID: WP_011251286.1
• Coordinates: 184727..185146 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ73_RS26555 (HUZ73_26460)	180418..180975	-	558	WP_017900872.1	HutD family protein	-
HUZ73_RS26560 (HUZ73_26465)	180972..181733	-	762	WP_017900871.1	histidine utilization repressor	-
HUZ73_RS26565 (HUZ73_26470)	181836..183203	+	1368	WP_023329010.1	formimidoylglutamate deiminase	-
HUZ73_RS26570 (HUZ73_26475)	183612..184580	-	969	Protein_198	IS5 family transposase	-
HUZ73_RS26575 (HUZ73_26480)	184727..185146	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HUZ73_RS26580 (HUZ73_26485)	185143..185454	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUZ73_RS26585 (HUZ73_26490)	185659..186096	-	438	Protein_201	DDE-type integrase/transposase/recombinase	-
HUZ73_RS26590 (HUZ73_26495)	186210..186548	+	339	WP_023288266.1	transposase	-
HUZ73_RS26595 (HUZ73_26500)	186545..186892	+	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
HUZ73_RS26600 (HUZ73_26505)	186941..187990	+	1050	Protein_204	IS66 family transposase	-
HUZ73_RS26605 (HUZ73_26510)	188048..189015	-	968	Protein_205	IS5 family transposase	-
HUZ73_RS26610 (HUZ73_26515)	189059..189496	+	438	WP_135720512.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / blaLAP-2 / aph(6)-Id / floR / sul2 / aph(4)-Ia / aac(3)-IVa / aph(3')-Ia / aadA2 / dfrA12 / tet(A)	iutA / iucD / iucC / iucB / iucA / pla	1..242129	242129
	-	inside	IScluster/Tn	-	-	183612..195237	11625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T161135 WP_011251285.1 NZ_CP055034:c185454-185143 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T161135 NZ_CP071063:62125-62523 [Enterobacter mori]
ATGCTGCATATGCTGGACACAAACATCGTAAGCCATCTTGTGAGGCAACACCCCGAGGTTCTAAATCAATATTCGCGTAT
CGCGCCTGAAAAAATGTGTATTTCAAGCGTAACAGAGGCTGAATTACTTTACGGCGTAGCCAAAAAGCAAAACAACCAAC
TGCACAAAACCATACTGGAATTTCTAAAAACCATCACCATTTGCGACTGGGACAGTGAAGCCGCAGCCGCCTACGGCGAG
CTTCGTGCAGTGATGGAAAAGAAAGGGAAGGTGATGGGCGATCTCGACCAGTTGATTGCTGCGCATGCCATCAGCCGGGG
TACGACGATTGTCACCAACGATCGTGCATTTGGGATGGTTCAAGACCTTAGGGTAGAGGACTGGACTACCGCAGCCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT161135 WP_011251286.1 NZ_CP055034:c185146-184727 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT161135 NZ_CP071063:61861-62121 [Enterobacter mori]
ATGGAGCGCACAGTAAAACTGATCAAAAAAGGGCGAAAGCAGGCTGTTGTTCTGCCTGCTGAGTTTGCGTTTGATACGGA
GAGCGTTTACATCCGACGGGATGACGAGGGGAATATTGTTTTGACGGCAAGATCGGAAAAAGAGCGACACAGGGACAACT
TTTTGCGTTTGCTCAAACAGACGCATGTGCCGGATTCGTTTCTGAGCAAAGAGGAGCGTAATCAGAGCTATTCGACACGA
GATCCTCTGGAAGGGCTGTAG

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1