Detailed information of TA system TA161130

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4595646..4596337
• Replicon: chromosome
• Accession: NZ_CP055033
• Organism: Klebsiella pneumoniae strain SWHEFF_62

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A483YFJ1
• Locus tag: HUZ73_RS22645
• Protein ID: WP_025987721.1
• Coordinates: 4595646..4595987 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2A5MHA6
• Locus tag: HUZ73_RS22650
• Protein ID: WP_019725272.1
• Coordinates: 4596011..4596337 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ73_RS22610 (4590869)	4590869..4591204	-	336	WP_239068161.1	hypothetical protein	-
HUZ73_RS22615 (4591168)	4591168..4591812	-	645	WP_164972967.1	hypothetical protein	-
HUZ73_RS22620 (4591809)	4591809..4592501	-	693	WP_040173594.1	HNH endonuclease signature motif containing protein	-
HUZ73_RS22625 (4592698)	4592698..4593280	+	583	Protein_4434	tyrosine-type recombinase/integrase	-
HUZ73_RS22630 (4593436)	4593436..4594329	+	894	WP_040173596.1	hypothetical protein	-
HUZ73_RS22635 (4594408)	4594408..4594692	+	285	Protein_4436	helix-turn-helix domain-containing protein	-
HUZ73_RS22640 (4594698)	4594698..4595531	-	834	WP_040173641.1	DUF4942 domain-containing protein	-
HUZ73_RS22645 (4595646)	4595646..4595987	-	342	WP_025987721.1	TA system toxin CbtA family protein	Toxin
HUZ73_RS22650 (4596011)	4596011..4596337	-	327	WP_019725272.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ73_RS22655 (4596351)	4596351..4596827	-	477	WP_019725273.1	DNA repair protein RadC	-
HUZ73_RS22660 (4596837)	4596837..4597283	-	447	WP_040173599.1	antirestriction protein	-
HUZ73_RS22665 (4597494)	4597494..4598183	-	690	WP_009309812.1	hypothetical protein	-
HUZ73_RS22670 (4598748)	4598748..4599638	-	891	WP_085163516.1	YfjP family GTPase	-
HUZ73_RS22675 (4599721)	4599721..4600809	-	1089	WP_085163515.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4590869..4617501	26632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12867.90 Da
• Isoelectric Point: 8.5195

>T161130 WP_025987721.1 NZ_CP055033:c4595987-4595646 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Nucleotide

• Length: 342 bp

>T161130 NZ_CP071062:1882516-1882830 [Dickeya solani]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGCTGTTTACCCGCTGTTGCTCGATGTCACAAGCGATATTATTGG
GCAGTTGAATCGCCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTATCCGGCAGGCCGCCGACCAGATCGTCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAGTACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTGCAGGCACTGGGG
GCGGTGTTTTGTGATGCGTCTCAGTATCGTACTCAGGTAAAGGCCGCAATAGATTTCCTCATCGACGGCATTTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12276.01 Da
• Isoelectric Point: 6.9560

>AT161130 WP_019725272.1 NZ_CP055033:c4596337-4596011 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT161130 NZ_CP071062:1882277-1882513 [Dickeya solani]
ATGACCGCGAAACAACGCAACACGCAAAGCGTGACCATGACGGTGGAGCGTGCCTTACTGGTCCGGGCGCGTGAAGCGGG
CATTAACCTGAGTGCCATCCTGACAACCGCCCTGGATGCAGAGCTTCGCCACCATGAAGCAAAAAAATGGCAGGAAGAGA
ACAGGGAAGCCATCGACGCATTAAACCGTTTTCATGATGAAAATGGCTGTTTCAGTGATGAATACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: A0A483YFJ1

Antitoxin

• AlphaFold DB: A0A2A5MHA6