Detailed information of TA system TA160955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 42317..42839
• Replicon: plasmid unnamed2
• Accession: NZ_CP055007
• Organism: Klebsiella pneumoniae strain STIN_77

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: HUZ53_RS27130
• Protein ID: WP_000220561.1
• Coordinates: 42558..42839 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: HUZ53_RS27125
• Protein ID: WP_000121743.1
• Coordinates: 42317..42568 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ53_RS27105 (HUZ53_27055)	38481..38669	+	189	WP_000957857.1	hypothetical protein	-
HUZ53_RS27110 (HUZ53_27060)	38679..39878	+	1200	WP_000948429.1	IS91 family transposase	-
HUZ53_RS27115 (HUZ53_27065)	40049..40222	-	174	WP_239619756.1	hypothetical protein	-
HUZ53_RS27120 (HUZ53_27070)	40243..41082	-	840	WP_024129959.1	replication initiation protein	-
HUZ53_RS27125 (HUZ53_27075)	42317..42568	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
HUZ53_RS27130 (HUZ53_27080)	42558..42839	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ53_RS27135 (HUZ53_27085)	43078..43170	-	93	Protein_48	IS6 family transposase	-
HUZ53_RS27140 (HUZ53_27090)	43336..44535	-	1200	WP_000948429.1	IS91 family transposase	-
HUZ53_RS27145 (HUZ53_27095)	44545..44733	-	189	WP_000957857.1	hypothetical protein	-
HUZ53_RS27150 (HUZ53_27100)	44827..45522	+	696	WP_239619757.1	replication initiation protein	-
HUZ53_RS27155 (HUZ53_27105)	45526..45972	+	447	WP_001074384.1	hypothetical protein	-
HUZ53_RS27160 (HUZ53_27115)	46109..46462	+	354	WP_024129958.1	DNA distortion polypeptide 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	dfrA12 / aadA2 / qacE / sul1 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-IIa / mph(A) / aph(3')-Ia	-	1..66378	66378
	-	inside	IScluster/Tn	dfrA12 / aadA2 / qacE / sul1 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-IIa	-	7948..44535	36587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T160955 WP_000220561.1 NZ_CP055007:42558-42839 [Klebsiella pneumoniae]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T160955 NZ_CP070978:1924539-1924880 [Burkholderia pyrrocinia]
ATGCGAACCGTCGCGTATGAAATCCATTTCTTCAGCGAGCAAGTCGAGGCAGGCGTGTTTGCGTTGCCGAAGACGCTGAC
CGCCAGGTTCTTCGCACTCGCTGACCGTATGGAGCAGTACGGCCCGAATCTGGGCGAACCTCACACCAGGCCGATCGGCG
ACGGGCTGTATGAAATGCGCTTGAAGGGCCTGGAAGGTATCGCCCGTGTGTTTTACTGTGCGGTCGTCAACCGGCGGATC
GTCATGCTTCACTGCTTCGTGAAGAAGACGCAGAAAACGCCGCAGAAAGAGCTGGAAACCGCGCGCCGCCGATTGAAGGA
GATTCGTTATGGCAACCTATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT160955 WP_000121743.1 NZ_CP055007:42317-42568 [Klebsiella pneumoniae]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT160955 NZ_CP070978:1924867-1925154 [Burkholderia pyrrocinia]
ATGGCAACCTATAAGGCACTGCGCGAGCGCGCGTTGTCCGATCCGAAGGTACGCGCCGAATACGACCGGCTGAACCGCGA
GGAATTCGCGCTGCTCGACGCAATGCTGGCCGCCCGGCGAGCCGCCGGTCTTTCGCAAGCCGATGTGGCGGAGCGCATGG
GCACGAAGGCGCCTGCCGTCACGCGGCTCGAACGCGCGCTCGCGACAGGCCAGCACTCTCCATCGATCGAGACGCTGCGC
AAGTATGCAGCCGCGTGCGGAAAGAAACTCGTGATCTCTTTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301