Detailed information of TA system TA160953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 28186..28829
• Replicon: plasmid unnamed1
• Accession: NZ_CP055006
• Organism: Klebsiella pneumoniae strain STIN_77

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HUZ53_RS26390
• Protein ID: WP_117500374.1
• Coordinates: 28186..28602 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HUZ53_RS26395
• Protein ID: WP_117500375.1
• Coordinates: 28599..28829 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ53_RS26360 (HUZ53_26320)	23204..23539	+	336	WP_275106660.1	LysR substrate-binding domain-containing protein	-
HUZ53_RS26365 (HUZ53_26325)	23670..24008	-	339	WP_007374386.1	carboxymuconolactone decarboxylase family protein	-
HUZ53_RS26370 (HUZ53_26330)	24025..24594	-	570	WP_042936984.1	TetR/AcrR family transcriptional regulator	-
HUZ53_RS26375 (HUZ53_26335)	24778..25335	-	558	WP_007374384.1	OsmC family protein	-
HUZ53_RS26380 (HUZ53_26340)	25544..26566	-	1023	WP_117500372.1	DNA helicase UvrD	-
HUZ53_RS26385 (HUZ53_26345)	26551..28113	-	1563	WP_117500373.1	AAA family ATPase	-
HUZ53_RS26390 (HUZ53_26350)	28186..28602	-	417	WP_117500374.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ53_RS26395 (HUZ53_26355)	28599..28829	-	231	WP_117500375.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUZ53_RS26400 (HUZ53_26360)	29329..29631	+	303	WP_047366185.1	hypothetical protein	-
HUZ53_RS26405 (HUZ53_26365)	29682..30389	+	708	WP_052685521.1	hypothetical protein	-
HUZ53_RS26410 (HUZ53_26370)	30431..31174	+	744	WP_047366186.1	site-specific integrase	-
HUZ53_RS26415 (HUZ53_26375)	31365..31619	+	255	Protein_38	site-specific integrase	-
HUZ53_RS26420 (HUZ53_26380)	31866..32876	-	1011	WP_045334592.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..115763	115763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15164.59 Da
• Isoelectric Point: 7.2455

>T160953 WP_117500374.1 NZ_CP055006:c28602-28186 [Klebsiella pneumoniae]
VNRIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKAAPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T160953 NZ_CP070978:c1001132-1000725 [Burkholderia pyrrocinia]
ATGAAGTTTCTGCTGGATACCAACGCGGTAATCGCCATTCTCAAAGGCGAGCCGGCCATGCTGGCCAAGCTGCGTGCGCA
TCGACCGGCAGACTTCGGAATTCCGGCGATCGTCGCGCATGAGCTCTATTACGGCGCTTACAAAAGTCGGCGAGTGGCAG
CCAATCTGGCACGCGTGGAGGGGCTTCAGTTCGAGGTCGTGTCGTTCGACGCGGAAGATGCTCAGCATGCCGGCGAAATT
CGTGCGCATTGGACCGCGGCAGGAACACCGATCGGCCCATATGATGCATTGATCGCAGGGCAGGCGCGTGCGCGGCGGCT
GGTGCTTGTCACGCATGACATGCGTGAATTCGAGCGAGTCCCGGAGCTGCAGATCGAAGATTGGCTCGCAGGCGCGCACG
AAAACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8660.70 Da
• Isoelectric Point: 4.3322

>AT160953 WP_117500375.1 NZ_CP055006:c28829-28599 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLENADPDFMAEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT160953 NZ_CP070978:c1001371-1001129 [Burkholderia pyrrocinia]
ATGGATATTGCCAAGATCTTCAAGCATGGCGGATCGCAGGCCGTCCGGCTGCCGAAGGATTTCCGCTTCGAGACGACCGA
GGTCCGGATTCGCCGCCACGGGGCGTCCGTCATTCTCGAGCCGGTGCCGCAAGACTGGGCATGGCTGACACCGCTGATCG
GCCCGGTCGACGCCGACTTCGAGGCCGCTGCAACAACCCAGCCGGACGTTCAAGAGCGCCCGGGACTGGACGTGTTTGAA
TGA